Amyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cells

Abstract

Amyloid precursor protein binding protein-1 (APP-BP1) was first identified as an interacting protein of APP. In this study, we explored whether APP-BP1 plays a role in neuronal differentiation of fetal neural stem cells. APP-BP1 knockdown by small interfering RNA treatment was found to downregulate neuronal differentiation and to upregulate APP intracellular domain production from APP in fetal neural stem cells. Furthermore, the change in gene expression profiles was systemically examined by DNA microarray. The expression of several genes including ephrin A2 was upregulated by APP-BP1 knockdown as assessed with DNA microarray and reverse transcriptase-polymerase chain reaction. Taken together, our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells. NeuroReport 23:61-66 (C) 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.