DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, KM | - |
dc.contributor.author | Jeong, E | - |
dc.contributor.author | Jeon, W | - |
dc.contributor.author | Cho, M | - |
dc.contributor.author | Ban, C | - |
dc.date.accessioned | 2016-03-31T09:12:45Z | - |
dc.date.available | 2016-03-31T09:12:45Z | - |
dc.date.created | 2012-03-06 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.other | 2011-OAK-0000024798 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16827 | - |
dc.description.abstract | A gold nanoparticle based dual fluorescence-colorimetric method was developed as an aptasensor to detect ampicillin using its single-stranded DNA (ssDNA) aptamer, which was discovered by a magnetic bead-based SELEX technique. The selected aptamers, AMP4 (5'-CACGGCATGGTGGGCGTCGTG-3'), AMP17 (5'-GCGGGCGGTTGTATAGCGG-3'), and AMP18 (5'-TTAGTTGGGGTTCAGTTGG-3'), were confirmed to have high sensitivity and specificity to ampicillin (K (d), AMP7 = 9.4 nM, AMP17 = 13.4 nM, and AMP18 = 9.8 nM, respectively). The 5'-fluorescein amidite (FAM)-modified aptamer was used as a dual probe for observing fluorescence differences and color changes simultaneously. The lower limits of detection for this dual method were a 2 ng/mL by fluorescence and a 10 ng/mL by colorimetry for ampicillin in the milk as well as in distilled water. Because these detection limits were below the maximum residue limit of ampicillin, this aptasensor was sensitive enough to detect antibiotics in food products, such as milk and animal tissues. In addition, this dual aptasensor will be a more accurate method for antibiotics in food products as it concurrently uses two detection methods: fluorescence and colorimetry. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Springer | - |
dc.relation.isPartOf | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.subject | Ampicillin | - |
dc.subject | ssDNA aptamer | - |
dc.subject | Gold nanoparticle | - |
dc.subject | Fluorescence | - |
dc.subject | Colorimetry | - |
dc.subject | Aptasensor | - |
dc.subject | IN-VITRO SELECTION | - |
dc.subject | DNA APTAMERS | - |
dc.subject | RNA APTAMERS | - |
dc.subject | CHLORAMPHENICOL | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | BIOSENSOR | - |
dc.subject | RESIDUES | - |
dc.subject | DELIVERY | - |
dc.subject | TARGETS | - |
dc.subject | PROBES | - |
dc.title | Aptasensor for ampicillin using gold nanoparticle-based dual fluorescence-colorimetric methods | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1007/S00216-011-5662-3 | - |
dc.author.google | Song K.-M., Jeong E., Jeon W., Cho M., Ban C. | - |
dc.relation.volume | 402 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 1 | - |
dc.relation.lastpage | 9 | - |
dc.contributor.id | 10085220 | - |
dc.relation.journal | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.402, no.6, pp.1 - 9 | - |
dc.identifier.wosid | 000300308400017 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 9 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 402 | - |
dc.contributor.affiliatedAuthor | Ban, C | - |
dc.identifier.scopusid | 2-s2.0-84858705233 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 69 | - |
dc.description.scptc | 64 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VITRO SELECTION | - |
dc.subject.keywordPlus | DNA APTAMERS | - |
dc.subject.keywordPlus | RNA APTAMERS | - |
dc.subject.keywordPlus | CHLORAMPHENICOL | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | RESIDUES | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | TARGETS | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordAuthor | Ampicillin | - |
dc.subject.keywordAuthor | ssDNA aptamer | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Fluorescence | - |
dc.subject.keywordAuthor | Colorimetry | - |
dc.subject.keywordAuthor | Aptasensor | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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