DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, HH | - |
dc.contributor.author | Moon, PG | - |
dc.contributor.author | Lee, JE | - |
dc.contributor.author | Kim, JG | - |
dc.contributor.author | Lee, W | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Baek, MC | - |
dc.date.accessioned | 2016-03-31T09:17:51Z | - |
dc.date.available | 2016-03-31T09:17:51Z | - |
dc.date.created | 2012-01-16 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 2011-OAK-0000024517 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16968 | - |
dc.description.abstract | Rosiglitazone, one of the thiazolidinedione (TZD), is an oral antidiabetic drug that activates a gamma isoform of peroxisome proliferator-activated receptor (PPAR gamma). To identify target proteins induced by rosiglitazone in adipocytes, we first performed simultaneous in-depth proteomic profiling of cytosolic proteins and secreted proteins (secretome) from 3T3-L1 adipocytes using a label-free quantification method with nano-UPLC MS/MS. In total, we identified 646 proteins from 3T3-L1 adipocytes, of which 172 and 162 proteins were upregulated and downregulated > 1.5-fold, respectively, in rosiglitazone-treated cells, as compared to controls. Some differentially expressed proteins in particular, including fatty acid translocase (FAT)/CD36, fatty acid binding protein, lipoprotein lipase, acetyl CoA acyltransferase, carnitine O-palmitoyltransferase 2, sterol carrier protein, adiponectin, and phosphoenolpyruvate carboxykinase could explain the current action mechanism of TZDs. Furthermore, this study is the first to report on two potential target proteins of rosiglitazone, such as adenomatosis polyposis coli 2 (APC2), and eukaryotic translation initiation factor 5A-1 (eIF5A) related to apoptosis and cell division. Our data clearly suggest that in-depth proteomic approaches using cytosolic and secreted proteins are important and necessary for identification of drug targets at the protein level. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | The korea science Technology Center | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | 3T3-L1 adipocytes | - |
dc.subject | nano-UPLC MS/MS | - |
dc.subject | proteomic analysis | - |
dc.subject | rosiglitazone | - |
dc.subject | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject | ENZYME GENE-EXPRESSION | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | DIABETES-MELLITUS | - |
dc.subject | PEROXISOME | - |
dc.subject | THIAZOLIDINEDIONES | - |
dc.subject | METABOLISM | - |
dc.subject | OBESITY | - |
dc.subject | QUANTIFICATION | - |
dc.title | Identification of the target proteins of rosiglitazone in 3T3-L1 adipocytes through proteomic analysis of cytosolic and secreted proteins | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1007/S10059-011-0026-6 | - |
dc.author.google | Hwang, HH | - |
dc.author.google | Moon, PG | - |
dc.author.google | Lee, JE | - |
dc.author.google | Kim, JG | - |
dc.author.google | Lee, W | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Baek, MC | - |
dc.relation.volume | 31 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 239 | - |
dc.relation.lastpage | 246 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.31, no.3, pp.239 - 246 | - |
dc.identifier.wosid | 000288805400006 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 246 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 239 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-79960016927 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 13 | - |
dc.description.scptc | 13 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | PEROXISOME | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordAuthor | 3T3-L1 adipocytes | - |
dc.subject.keywordAuthor | nano-UPLC MS/MS | - |
dc.subject.keywordAuthor | proteomic analysis | - |
dc.subject.keywordAuthor | rosiglitazone | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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