Guest Binding Dynamics with Cucurbit[7]uril in the Presence of Cations
SCIE
SCOPUS
- Title
- Guest Binding Dynamics with Cucurbit[7]uril in the Presence of Cations
- Authors
- Tang, H; Fuentealba, D; Ko, YH; Selvapalam, N; Kim, K; Bohne, C
- Date Issued
- 2011-12-21
- Publisher
- AMER CHEMICAL SOC
- Abstract
- The binding dynamics of R-(+)2-naphthyl-1-ethylammonium cation (NpH+) with cucurbit[7]uril (CB[7]) was investigated. Competitive binding with Na+ or H3O+ cations enabled the reaction to be slowed down sufficiently for the kinetics to be studied by fluorescence stopped-flow experiments. The binding of two Na+ cations to CB[7], i.e., CB[7]center dot Na+ (K-01 = 130 +/- 10 M-1) and Na+ center dot CB[7] center dot Na+ (K-02 = 21 +/- 2 M-1), was derived from the analysis of binding isotherms and the kinetic studies. NpH+ binds only to free CB [7] ((1.06 +/- 0.05) x 10(7) M-1) and the association rate constant of (6.3 +/- 0.3) x 10(8) M-1 s(-1) is 1 order of magnitude lower than that for a diffusion-controlled process and much higher than the association rate constant previously determined for other CB[n] systems. The high equilibrium constant for the NpH+@CB[7] complex is a consequence of the slow dissociation rate constant of SS s(-1). The kinetics results showed that formation of a complex between a positively charged guest with CB[n] can occur at a rate close to the diffusion-controlled limit with no detection of a stable exclusion complex.
- Keywords
- TANDEM ENZYME ASSAYS; HOST-GUEST; BETA-CYCLODEXTRIN; AQUEOUS-SOLUTION; PK(A) SHIFTS; CUCURBITURIL HOMOLOGS; COMPLEXATION DYNAMICS; INCLUSION-COMPOUNDS; PROTEIN-LIGAND; DRUG-DELIVERY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17032
- DOI
- 10.1021/JA209266X
- ISSN
- 0002-7863
- Article Type
- Article
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 50, page. 20623 - 20633, 2011-12-21
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