Nanoparticle-based Indicator-Displacement Assay for Pyrophosphate
SCIE
SCOPUS
- Title
- Nanoparticle-based Indicator-Displacement Assay for Pyrophosphate
- Authors
- Oh, DJ; Kim, KM; Ahn, KH
- Date Issued
- 2011-08-01
- Publisher
- WILEY-BLACKWELL
- Abstract
- Silica nanoparticles functionalized with a Zn(II)-dipicolylamine derivative are used as the receptor component of a sensing ensemble for an indicator-displacement assay. The nano-ensemble system, constructed with pyrocatechol violet as an indicator, selectively senses pyrophosphate over other anions including hydrogen phosphate, thus showing a visible blue-to-yellow color change and the corresponding absorption spectral changes in a buffer solution of pH 7. A distinct recognition behavior-the nano-ensemble does not sense hydrogen phosphate that was sensed by the traditional ensemble with the same molecular receptor-is explained by an incomplete-displacement model in the case of the integrated receptor system. The present work demonstrates that the nanoparticle-based competition assay is as effective as the traditional approach with molecular receptors. Furthermore, such an integrated receptor system can provide distinct recognition behavior from its molecular receptor.
- Keywords
- indicator-displacement assay; nanoparticles; receptors; supramolecular chemistry; zinc; CHEMOSENSING ENSEMBLE; HIGH-AFFINITY; PHYSIOLOGICAL CONDITIONS; FLUORESCENT DETECTION; HIGH SELECTIVITY; PHOSPHATE ANION; WATER; RECEPTOR; RECOGNITION; BEVERAGES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17093
- DOI
- 10.1002/ASIA.201100149
- ISSN
- 1861-4728
- Article Type
- Article
- Citation
- CHEMISTRY-AN ASIAN JOURNAL, vol. 6, no. 8, page. 2033 - 2038, 2011-08-01
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