Quantification of Tryptic Peptides in Quadrupole Ion Trap Using High-Mass Signals Derived from Isotope-Coded N-Acetyl Dipeptide Tags
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SCOPUS
- Title
- Quantification of Tryptic Peptides in Quadrupole Ion Trap Using High-Mass Signals Derived from Isotope-Coded N-Acetyl Dipeptide Tags
- Authors
- SEO, JONGCHEOL; Yoon, HJ; Shin, SK
- Date Issued
- 2011-09
- Publisher
- SPRINGER
- Abstract
- Isotope-labeled N-acetyl dipeptides (Ac-Xxx-Ala) are coupled to the primary amines of tryptic peptides and then analyzed by tandem mass spectrometry. Amide bond cleavage between Xxx and Ala provides both low- and high-mass isotope-coded signals for quantification of peptides. Especially, facile cleavage at the modified lysine side chain yields very strong high-mass quantitation signals in a noise-free region. Tagging tryptic peptides with isobaric N-acetyl dipeptides is a viable strategy for accurate quantification of proteins, which can be used with most quadrupole ion trap mass spectrometers carrying the 1/3 mass cut-off problem.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17094
- DOI
- 10.1007/S13361-011-0189-6
- ISSN
- 1044-0305
- Article Type
- Article
- Citation
- JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 22, no. 9, page. 1668 - 1677, 2011-09
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