DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, K | - |
dc.contributor.author | Hong, SW | - |
dc.contributor.author | Hur, W | - |
dc.contributor.author | Lee, MY | - |
dc.contributor.author | Yang, JA | - |
dc.contributor.author | Kim, SW | - |
dc.contributor.author | Yoon, SK | - |
dc.contributor.author | Hahn, SK | - |
dc.date.accessioned | 2016-03-31T09:32:50Z | - |
dc.date.available | 2016-03-31T09:32:50Z | - |
dc.date.created | 2011-07-11 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 2011-OAK-0000023812 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17323 | - |
dc.description.abstract | A target specific systemic delivery system of siRNA therapeutics was successfully developed using reducible polyethyleneimine grafted hyaluronic acid [(PEI-SS)-g-HA] conjugates. The PEI-SS was synthesized by Michael addition of low molecular weight PEI (MW = 2000) with cystaminebisacrylamide (CBA), and grafted to carboxyl groups of HA via amide bond formation after activation with 1-ethy1-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 1-hydroxybenzotriazole monohydrate (HOBt). The confocal microscopic and fluorometric analyses confirmed the effective cellular uptake of siRNA/(PEI-SS)-g-HA complex by HA receptor mediated endocytosis. In vitro gene silencing efficiency was ca. 80% in the presence of 10 vol % serum and ca. 50% in the presence of 50 vol% serum in B16F1 melanoma cells and activated hepatic stellate cells (HSCs). Furthermore, target specific systemic delivery of apolipoprotein B (ApoB) siRNA/(PEI-SS)-g-HA complex resulted in a drastically reduced ApoB mRNA level down to ca. 20% in a dose-dependent manner. Finally, TGF-beta siRNA/(PEI-SS)-g-HA complex showed a feasible therapeutic effect on liver cirrhosis with a significantly reduced nodule formation, collagen content, and HSC number. The siRNA/(PEI-SS)-g-HA complex can be exploited for the target specific systemic treatment of various liver diseases. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.subject | Hyaluronic acid | - |
dc.subject | Polyethyleneimine | - |
dc.subject | siRNA | - |
dc.subject | Targeted systemic delivery | - |
dc.subject | Liver cirrhosis | - |
dc.subject | HYALURONIC-ACID | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | RECEPTOR | - |
dc.subject | FIBROSIS | - |
dc.subject | SIRNA | - |
dc.subject | NANOPARTICLES | - |
dc.subject | ACTIVATION | - |
dc.subject | CONJUGATE | - |
dc.subject | THERAPY | - |
dc.subject | CD44 | - |
dc.title | Target specific systemic delivery of TGF-beta siRNA/(PEI-SS)-g-HA complex for the treatment of liver cirrhosis | - |
dc.type | Article | - |
dc.contributor.college | 신소재공학과 | - |
dc.identifier.doi | 10.1016/J.BIOMATERIALS.2011.03.044 | - |
dc.author.google | Park, K | - |
dc.author.google | Hong, SW | - |
dc.author.google | Hur, W | - |
dc.author.google | Lee, MY | - |
dc.author.google | Yang, JA | - |
dc.author.google | Kim, SW | - |
dc.author.google | Yoon, SK | - |
dc.author.google | Hahn, SK | - |
dc.relation.volume | 32 | - |
dc.relation.issue | 21 | - |
dc.relation.startpage | 4951 | - |
dc.relation.lastpage | 4958 | - |
dc.contributor.id | 10149037 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.32, no.21, pp.4951 - 4958 | - |
dc.identifier.wosid | 000291571400028 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 4958 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 4951 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-79955780014 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 37 | - |
dc.description.scptc | 38 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HYALURONIC-ACID | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CONJUGATE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CD44 | - |
dc.subject.keywordAuthor | Hyaluronic acid | - |
dc.subject.keywordAuthor | Polyethyleneimine | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Targeted systemic delivery | - |
dc.subject.keywordAuthor | Liver cirrhosis | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
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