DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DW | - |
dc.contributor.author | Son, S | - |
dc.contributor.author | Jang, J | - |
dc.contributor.author | Youn, H | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | Lee, D | - |
dc.contributor.author | Lee, YS | - |
dc.contributor.author | Jeong, JM | - |
dc.contributor.author | Kim, WJ | - |
dc.contributor.author | Lee, DS | - |
dc.date.accessioned | 2016-03-31T09:34:29Z | - |
dc.date.available | 2016-03-31T09:34:29Z | - |
dc.date.created | 2011-07-11 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 2011-OAK-0000023771 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17353 | - |
dc.description.abstract | Recent advances in efficient microRNA (miRNA) delivery techniques using brain-targeted nanoparticles offer critical information for understanding the functional role of miRNAs in vivo, and for supporting targeted gene therapy in terms of treating miRNA-associated neurological diseases. Here, we report the rabies virus glycoprotein (RVG)-labeled non-toxic SSPEI nanomaterials capable of neuron-specific miR-124a delivery to neuron in vivo. The RVG-labeled BPEI-SS (RVG-SSPEI) nanocarrier showed less toxicity in acetylcholine receptor-positive Neuro2a cells, and electrostatic interaction of RVG-SSPEI with miR-124a exhibited optimal transfection efficacy. The RVG-SSPEI polymer specifically targeted Neuro2a using cy5.5-miR-124a mixed with RVG-SSPEI. The functional action of miR-124a oligomers released from polyplexes in the cytoplasmic region was evaluated by a reporter vector containing a miR-124a -binding sequence, and showed a significantly reduced reporter signal in a dose-dependent manner. Cy5.5-miR-124a/RVG-SSPEI- injected into mice via tail veins displayed the enhanced accumulation of miR-124a in the isolated brain. Hindrance of the efficient penetration of neuronal cells by size limitation of the miR-124a/RVG-SSPEI improved with the help of mannitol through blood-brain barrier disruption. These findings indicated that the RVG peptide combined with mannitol infusion using SSPEI polymer for neuron-specific targeting in vivo is sufficient to deliver neurogenic microRNA into the brain. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.subject | MicroRNA | - |
dc.subject | RVG peptide | - |
dc.subject | PEI polymer | - |
dc.subject | Brain-targeting agent | - |
dc.subject | Polyplex | - |
dc.subject | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject | NEURONAL DIFFERENTIATION | - |
dc.subject | RNA INTERFERENCE | - |
dc.subject | SIRNA | - |
dc.subject | NANOPARTICLES | - |
dc.subject | MIR-124 | - |
dc.subject | VECTOR | - |
dc.subject | EXPRESSION | - |
dc.subject | TOXICITY | - |
dc.subject | MICE | - |
dc.title | A brain-targeted rabies virus glycoprotein-disulfide linked PEI nanocarrier for delivery of neurogenic microRNA | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/J.BIOMATERIALS.2011.03.047 | - |
dc.author.google | Hwang, DW | - |
dc.author.google | Son, S | - |
dc.author.google | Jang, J | - |
dc.author.google | Youn, H | - |
dc.author.google | Lee, S | - |
dc.author.google | Lee, D | - |
dc.author.google | Lee, YS | - |
dc.author.google | Jeong, JM | - |
dc.author.google | Kim, WJ | - |
dc.author.google | Lee, DS | - |
dc.relation.volume | 32 | - |
dc.relation.issue | 21 | - |
dc.relation.startpage | 4968 | - |
dc.relation.lastpage | 4975 | - |
dc.contributor.id | 10135304 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.32, no.21, pp.4968 - 4975 | - |
dc.identifier.wosid | 000291571400030 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 4975 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 4968 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Kim, WJ | - |
dc.identifier.scopusid | 2-s2.0-79955774513 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 127 | - |
dc.description.scptc | 139 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | VECTOR | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | MicroRNA | - |
dc.subject.keywordAuthor | RVG peptide | - |
dc.subject.keywordAuthor | PEI polymer | - |
dc.subject.keywordAuthor | Brain-targeting agent | - |
dc.subject.keywordAuthor | Polyplex | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
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