Interaction between glutathione and glutathione-S-transferase on dendron self-assembled controlled pore glass beads
SCIE
SCOPUS
- Title
- Interaction between glutathione and glutathione-S-transferase on dendron self-assembled controlled pore glass beads
- Authors
- Chen, LH; Choi, YS; Kwon, J; Wang, RS; Lee, T; Ryu, SH; Park, JW
- Date Issued
- 2004-08-16
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Abstract
- Controlled pore glass beads were modified with a conical shaped dendron and interaction between immobilized glutathione on top of the dendron and glutathione-S-transferase (GST) in cell lysate was examined. SDS-PAGE chromatogram showed that the dendron modified beads were effective not only in binding the corresponding proteins such as GST and GST-fused proteins but also in suppressing the nonspecific binding of proteins. Dramatic increase of ligand utilization (ca. 30%) was observed in comparison with the conventional approaches. Molecular weight dependence of the beads is comparable to that of Sepharose 4B. (C) 2004 Elsevier Ltd. All rights reserved.
- Keywords
- glutathione; glutathione-S-transferase; controlled pore glass; dendron; DODECYL SULFATE COMPLEXES; AFFINITY-CHROMATOGRAPHY; ESCHERICHIA-COLI; MOLECULAR-WEIGHT; SPACER ARMS; THIN-FILMS; ADSORPTION; PROTEINS; PURIFICATION; RESIST
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17773
- DOI
- 10.1016/j.tet.2004.05.043
- ISSN
- 0040-4020
- Article Type
- Article
- Citation
- TETRAHEDRON, vol. 60, no. 34, page. 7293 - 7299, 2004-08-16
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- There are no files associated with this item.
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