DC Field | Value | Language |
---|---|---|
dc.contributor.author | Irino, Y | - |
dc.contributor.author | Cho, HY | - |
dc.contributor.author | Nakamura, Y | - |
dc.contributor.author | Nakahara, M | - |
dc.contributor.author | Furutani, M | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Takenawa, T | - |
dc.contributor.author | Fukami, K | - |
dc.date.accessioned | 2016-03-31T12:21:45Z | - |
dc.date.available | 2016-03-31T12:21:45Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2004-07-23 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.other | 2004-OAK-0000004393 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17830 | - |
dc.description.abstract | To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, beta-, gamma-, delta-, epsilon- and zeta-type. PLCdelta-type is reported to be composed of four isozymes, PLCdelta1-delta4. Here we report that a screening for mouse PLCdelta2 from a BAC library with primers that amplify a specific region of bovine PLCdelta2 resulted in isolation of one clone containing the mouse PLCdelta4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCdelta2 and PLCdelta4 in the mouse and human genomes, indicating that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4. However, PLCdelta2 Western blot analysis with a widely used commercial anti-PLCdelta2 antibody showed all expression pattern distinct from that of PLCdelta4 in wildtype mice. In addition, an 80-kDa band, which was recognized by antibody against PLCdelta2, was smaller than an 85-kDa band detected by anti-PLCdelta4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCdelta4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCdelta2 antibody contained no PLC activity. From these data, we conclude that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4, and that three isozymes (delta1, delta3, and delta4) exist in the PLCdelta family. (C) 2004 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.subject | phospholipase C | - |
dc.subject | PLC delta 2 | - |
dc.subject | PLC delta 4 | - |
dc.subject | mouse genome | - |
dc.subject | INOSITOL TRISPHOSPHATE | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | HYPERTENSIVE RATS | - |
dc.subject | ACROSOME REACTION | - |
dc.subject | MEMBRANE | - |
dc.subject | CELL | - |
dc.subject | DOMAIN | - |
dc.subject | EXPRESSION | - |
dc.subject | C-DELTA-4 | - |
dc.subject | EPSILON | - |
dc.title | Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4 | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1016/J.BBRC.2004. | - |
dc.author.google | Irino, Y | - |
dc.author.google | Cho, HY | - |
dc.author.google | Nakamura, Y | - |
dc.author.google | Nakahara, M | - |
dc.author.google | Furutani, M | - |
dc.author.google | Suh, PG | - |
dc.author.google | Takenawa, T | - |
dc.author.google | Fukami, K | - |
dc.relation.volume | 320 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 537 | - |
dc.relation.lastpage | 543 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.320, no.2, pp.537 - 543 | - |
dc.identifier.wosid | 000222567700039 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 543 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 537 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 320 | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 34 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INOSITOL TRISPHOSPHATE | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | HYPERTENSIVE RATS | - |
dc.subject.keywordPlus | ACROSOME REACTION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | C-DELTA-4 | - |
dc.subject.keywordPlus | EPSILON | - |
dc.subject.keywordAuthor | phospholipase C | - |
dc.subject.keywordAuthor | PLC delta 2 | - |
dc.subject.keywordAuthor | PLC delta 4 | - |
dc.subject.keywordAuthor | mouse genome | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
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