DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, IH | - |
dc.contributor.author | You, JO | - |
dc.contributor.author | Ha, KS | - |
dc.contributor.author | Bae, DS | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Rhee, SG | - |
dc.contributor.author | Bae, YS | - |
dc.date.accessioned | 2016-03-31T12:24:34Z | - |
dc.date.available | 2016-03-31T12:24:34Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2004-06-18 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2004-OAK-0000004328 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17880 | - |
dc.description.abstract | We have recently shown that phospholipase C-gamma (PLC-gamma) is activated by the central repeated units ( CRUs) of the AHNAK protein in the presence of arachidonic acid. Here we demonstrate that four central repeated units ( 4 CRUs) of AHNAK act as a scaffolding motif networking PLC-gamma and PKC-alpha. Specifically, 4 CRUs of AHNAK bind and activate PKC-alpha, which in turn stimulates the release of arachidonic acid near where PLC-gamma1 is localized. Moreover, 4 CRUs of AHNAK interacted with PLC-gamma and the concerted action of 4 CRUs with arachidonic acid stimulated PLC-gamma activity. Stimulation of NIH3T3 cells expressing 4 CRUs of AHNAK with phorbol 12-myristate 13-acetate resulted in the increased generation of total inositol phosphates (IPT) and mobilization of the intracellular calcium. Phorbol 12-myristate 13-acetate-dependent generation of IPT was completely blocked in NIH3T3 cells depleted of PLC-gamma1 by RNA interference. Furthermore, bradykinin, which normally stimulated the PLC-beta isozyme resulting in the generation of a monophasic IPT within 30 s in NIH3T3 cells, led to a biphasic pattern for generation of IPT in NIH3T3 cells expressing 4 CRUs of AHNAK. The secondary activation of PLC is likely because of the scaffolding activity of AHNAK, which is consistent with the role of 4 CRUs as a molecular linker between PLC-gamma and PKC-alpha. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | ARACHIDONIC-ACID RELEASE | - |
dc.subject | A(2) | - |
dc.subject | CA2+ | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | BRADYKININ | - |
dc.subject | CALCIUM | - |
dc.subject | GAMMA | - |
dc.subject | CELLS | - |
dc.subject | PLC-GAMMA-1 | - |
dc.subject | FIBROBLASTS | - |
dc.title | AHNAK-mediated activation of phospholipase C-gamma 1 through protein kinase C | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/JBC.M3115252 | - |
dc.author.google | Lee, IH | - |
dc.author.google | You, JO | - |
dc.author.google | Ha, KS | - |
dc.author.google | Bae, DS | - |
dc.author.google | Suh, PG | - |
dc.author.google | Rhee, SG | - |
dc.author.google | Bae, YS | - |
dc.relation.volume | 279 | - |
dc.relation.issue | 25 | - |
dc.relation.startpage | 26645 | - |
dc.relation.lastpage | 26653 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.279, no.25, pp.26645 - 26653 | - |
dc.identifier.wosid | 000222003000091 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 26653 | - |
dc.citation.number | 25 | - |
dc.citation.startPage | 26645 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 279 | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 37 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ARACHIDONIC-ACID RELEASE | - |
dc.subject.keywordPlus | A(2) | - |
dc.subject.keywordPlus | CA2+ | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | BRADYKININ | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | GAMMA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PLC-GAMMA-1 | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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