세포프린팅 기술 기반 간 조직 공학을 위한 혈관생성이 고려된 3차원 공생배양 인공지지체의 제작

Abstract

The aim of this study was to build a three-dimensional scaffold with micro vascular networks for liver tissue regeneration. Recently, the cell printing technology was utilized as a way of mimicking the microstructure of the original tissues. One crucial obstacle facing cell printing technology is the fabrication of cell-printed three-dimensional constructs because hydrogels, which have been used as the main material in cell printing area, is not able to maintain three-dimensional structure due to their poor mechanical properties. In this study, polycaprolactone(PCL), which has relatively high mechanical properties, was used as a frame work. Cell-laden collagen was then infused into previously prepared framework made of PCL. For this work, a multi-head deposition system(MHDS) was used to fabricate a PCL framework and simultaneously print collagen containing three different types of cells, which are hepatocytes, human umbilical vein endothelial cells(HUVECs), and human lung fibroblasts, for formation of vascular networks and liver tissue regeneration. From this experiment, triple cell-printed three dimensional structure were successfully fabricated. Further, vascular formation was confirmed up to 14 days via observation with confocal microscope and the functional abilities of hepatocytes, such as albumin secretion and urea synthesis, were checked up to 10 days after cell printing. Therefore, our researches showed the promising possibility for liver tissue engineering.