DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YJ | - |
dc.contributor.author | Kim, JE | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Lee, MH | - |
dc.contributor.author | Jung, HW | - |
dc.contributor.author | Bahk, YY | - |
dc.contributor.author | Hwang, BK | - |
dc.contributor.author | Hwang, I | - |
dc.contributor.author | Kim, WT | - |
dc.date.accessioned | 2016-03-31T12:38:42Z | - |
dc.date.available | 2016-03-31T12:38:42Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2004-01-02 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.other | 2004-OAK-0000003959 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18141 | - |
dc.description.abstract | Phosphoinositide-specific phospholipase C (PI-PLC) catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate inositol 1,4,5-trisphosphate and diacylglycerol, both of which act as secondary messengers in animal cells. In this report, we identified in Vigna radiata L. (mung bean) three distinct partial cDNAs (pVr-PLC1, pVr-PLC2, and pVrPLC3), which encode forms of putative PI-PLC. All three Vr-PLC genes were transcriptionally active and displayed unique patterns of expression. The Vr-PLC1 and Vr-PLC2 transcripts were constitutively expressed to varying degrees in every tissue of mung bean plants examined. In contrast, the Vr-PLC3 mRNA level was very low under normal growth conditions and was rapidly induced in an abscisic acid-independent manner under environmental stress conditions (drought and high salinity). An isolated genomic clone, about 8.2 kb in length, showed that Vr-PLC1 and Vr-PLC3 are in tandem array in the mung bean genome. The predicted primary sequence of Vr-PLC3 (M-r = 67.4 kDa) is reminiscent of the delta-isoform of animal enzymes which contain core sequences found in typical PI-PLCs, such as the catalytic domain comprising X and Y motifs, a lipid-binding C2 domain, and the less conserved EF-hand domain. Results of in vivo targeting experiment using a green fluorescent protein (GFP) showed that the GFP-Vr-PLC3 fusion protein was localized primarily to the plasma membrane of the Arabidopsis protoplast. The C2 domain was essential for Vr-PLC3 to be targeted to the plasma membrane. The possible biological functions of stress-responsive Vr-PLC3 in mung bean plants are discussed. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | FEBS LETTERS | - |
dc.subject | abiotic stress | - |
dc.subject | calcium | - |
dc.subject | differential gene expression | - |
dc.subject | inositol 1,4,5-trisphosphate | - |
dc.subject | phospholipase C | - |
dc.subject | plasma membrane | - |
dc.subject | Vigna radiata | - |
dc.subject | LENGTH CDNA MICROARRAY | - |
dc.subject | OXIDASE MESSENGER-RNA | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | ABSCISIC-ACID | - |
dc.subject | PLANT-CELLS | - |
dc.subject | HYPEROSMOTIC STRESS | - |
dc.subject | DROUGHT | - |
dc.subject | ISOFORMS | - |
dc.subject | CALCIUM | - |
dc.title | The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.) | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1016/S0014-5793(03)01388-7 | - |
dc.author.google | Kim, YJ | - |
dc.author.google | Kim, JE | - |
dc.author.google | Lee, JH | - |
dc.author.google | Lee, MH | - |
dc.author.google | Jung, HW | - |
dc.author.google | Bahk, YY | - |
dc.author.google | Hwang, BK | - |
dc.author.google | Hwang, I | - |
dc.author.google | Kim, WT | - |
dc.relation.volume | 556 | - |
dc.relation.issue | 1-3 | - |
dc.relation.startpage | 127 | - |
dc.relation.lastpage | 136 | - |
dc.contributor.id | 10078446 | - |
dc.relation.journal | FEBS LETTERS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.556, no.1-3, pp.127 - 136 | - |
dc.identifier.wosid | 000188125600024 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 136 | - |
dc.citation.number | 1-3 | - |
dc.citation.startPage | 127 | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 556 | - |
dc.contributor.affiliatedAuthor | Hwang, I | - |
dc.identifier.scopusid | 2-s2.0-0346725028 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 49 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | OXIDASE MESSENGER-RNA | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | ABSCISIC-ACID | - |
dc.subject.keywordPlus | ARABIDOPSIS GENES | - |
dc.subject.keywordPlus | DROUGHT | - |
dc.subject.keywordPlus | SALT | - |
dc.subject.keywordPlus | ETHYLENE | - |
dc.subject.keywordPlus | ISOFORMS | - |
dc.subject.keywordPlus | ROOTS | - |
dc.subject.keywordPlus | DEHYDRATION | - |
dc.subject.keywordAuthor | abiotic stress | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | differential gene expression | - |
dc.subject.keywordAuthor | inositol 1,4,5-trisphosphate | - |
dc.subject.keywordAuthor | phospholipase C | - |
dc.subject.keywordAuthor | plasma membrane | - |
dc.subject.keywordAuthor | Vigna radiata | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
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