A complete sequence of the pGA1611 binary vector

Abstract

We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577-bp T-DNA region consists of a 1987-bp ubiquitine promoter region, 45 bp for the multiple cloning site, a 253-bp nos terminator region, and the 2045-bp CaMV35S-hph-T7 chimaeric gene. The vector backbone (7004 bp) carries oriT, traJ, trfA, teM, tetR, and oriV An 892-bp RB region and the 489-bp LB region are also present. The T-DNA possesses 15 unique sites, six of which are at the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector.