Enhanced UV-visible absorbance detection in capillary electrophoresis using modified T-shaped post-column flow cell
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- Title
- Enhanced UV-visible absorbance detection in capillary electrophoresis using modified T-shaped post-column flow cell
- Authors
- Lim, K; Kim, S; Hahn, JH
- Date Issued
- 2002-02-20
- Publisher
- KOREAN CHEMICAL SOC
- Abstract
- The construction of the T-shaped post-column flow cell has been changed to enhance the practicability as a UV-visible absorbance detector for capillary electrophoresis. In this new design, a rectangular cube-shaped inner structure is employed, which completely fits the outer rectangular tubing. This arrangement has greatly facilitated the fabrication of the T-cells. In addition, the volume for the auxiliary flow has been dramatically reduced down to 300 muL, and its volume flow rate is optimized at 4.2 muL/min. The short optical path length in the sheath flows (500 mum on each side) minimizes back-ground absorption, and thus enhances its performance in low-UV wavelengths. We have optimized the auxiliary flow rate at 50 mum/s, so that migration times are insensitive to the flow rate. This optimization has improved repeatabilities in migration times and peak heights. A double-beam detection scheme using a pair of photodiodes is employed to increase the signal-to-noise ratio.
- Keywords
- capillary electrophoresis; post-column; absorbance detection; extended path length; ZONE-ELECTROPHORESIS; ABSORBENCY DETECTION; FLUORESCENCE DETECTION; ABSORPTION DETECTION; SEPARATION; SENSITIVITY; MOLECULES; SYSTEMS; ENZYME
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19184
- DOI
- 10.5012/bkcs.2002.23.2.295
- ISSN
- 0253-2964
- Article Type
- Article
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, vol. 23, no. 2, page. 295 - 300, 2002-02-20
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