DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, SJ | - |
dc.contributor.author | Cho, SJ | - |
dc.contributor.author | Kim, CO | - |
dc.contributor.author | Park, JW | - |
dc.date.accessioned | 2016-03-31T13:09:12Z | - |
dc.date.available | 2016-03-31T13:09:12Z | - |
dc.date.created | 2009-03-16 | - |
dc.date.issued | 2002-03-05 | - |
dc.identifier.issn | 0743-7463 | - |
dc.identifier.other | 2002-OAK-0000002491 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19187 | - |
dc.description.abstract | Four kinds of aminosilane layers on glass slides or silicon wafers were prepared. The amine densities of the layers prepared with aminopropylmonoethoxydimethylsilane (APDES), aminopropylmonoethoxydimethylsilane (APMES), a mixture of (aminopropyl)triethoxysilane (APTES) and n-butyltrimethoxysilane (n-BTMS) (v/v = 1:10) are 4.0(+/-0.8), 1.0(+/-0.2), and 0.30(+/-0.6) amine/nm(2), respectively. A substrate with much higher amine density, that is, 40(+/-8) amines/nm(2) was also prepared by allowing aziridine to polymerize on the APDES-treated substrate. AFM revealed that APDES-, APMES-, and APTES/n-BTMS-treated surfaces were relatively flat; on the other hand, an aziridine-treated surface showed embossed morphology. The amine substrates were allowed to react with a heterobifunctional linker succinimidyl 4-maleimido butyrate (SMB), and subsequently pentadecadeoxynucleotides were microarrayed on the SMB-treated substrates. Characteristics of the DNA microarrays including the dynamic range, the mismatch discrimination efficiency, and so forth were examined. Noteworthily, DNA microarrays on the aziridine-polymerized substrate showed much higher fluorescence intensity. At the same time, DNA microarrays from these four substrates were able to discriminate internal- and terminal-mismatched pairs, but the fluorescence ratio was far from the one that thermodynamics implies. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | LANGMUIR | - |
dc.subject | DOUBLE-STRANDED-DNA | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | ATOMIC-FORCE MICROSCOPY | - |
dc.subject | COVALENT IMMOBILIZATION | - |
dc.subject | DSDNA MICROARRAYS | - |
dc.subject | SURFACE-DENSITY | - |
dc.subject | GOLD ELECTRODES | - |
dc.subject | GLASSY-CARBON | - |
dc.subject | THIN-LAYERS | - |
dc.subject | AMINE GROUP | - |
dc.title | Characteristics of DNA microarrays fabricated on various aminosilane layers | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1021/LA0113522 | - |
dc.author.google | Oh, SJ | - |
dc.author.google | Cho, SJ | - |
dc.author.google | Kim, CO | - |
dc.author.google | Park, JW | - |
dc.relation.volume | 18 | - |
dc.relation.issue | 5 | - |
dc.relation.startpage | 1764 | - |
dc.relation.lastpage | 1769 | - |
dc.contributor.id | 10053210 | - |
dc.relation.journal | LANGMUIR | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | LANGMUIR, v.18, no.5, pp.1764 - 1769 | - |
dc.identifier.wosid | 000174130500047 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1769 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1764 | - |
dc.citation.title | LANGMUIR | - |
dc.citation.volume | 18 | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.identifier.scopusid | 2-s2.0-0037023084 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 122 | - |
dc.description.scptc | 125 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SURFACE CHARACTERIZATION | - |
dc.subject.keywordPlus | COVALENT IMMOBILIZATION | - |
dc.subject.keywordPlus | DSDNA MICROARRAYS | - |
dc.subject.keywordPlus | GOLD ELECTRODES | - |
dc.subject.keywordPlus | GLASSY-CARBON | - |
dc.subject.keywordPlus | THIN-LAYERS | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | SILICON | - |
dc.subject.keywordPlus | MONOLAYERS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
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