Cross-priming as a predominant mechanism for inducing CD8(+) T cell responses in gene gun DNA immunization
SCIE
SCOPUS
- Title
- Cross-priming as a predominant mechanism for inducing CD8(+) T cell responses in gene gun DNA immunization
- Authors
- Cho, JH; Youn, JW; Sung, YC
- Date Issued
- 2001-11-15
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Abstract
- DNA immunization induces CD8(+) CTL responses by bone marrow-derived APCs, which are directly transfected with a plasmid DNA and/or acquire Ags from DNA-transfected non-APCs. To investigate the relative contribution of DNA-transfected APCs vs non-APCs to the initiation of CD8(+) T cell responses, we used tissue-specific promoter-directed gene expression and adoptive transfer systems in gene gun DNA immunization. In this study, we demonstrated that non-APC-specific gene expressions induced significant CD8(+) CTL and IFN-gamma -producing cells and Ab responses, whereas APC-specific gene expressions led to moderate CTL and IFN-gamma -producers, but no Ab responses. Interestingly, mice immunized with a non-A-PC-specific plasmid induced more rapid, vigorous, and prolonged proliferation of adoptively transferred Ag-specific CD8(+) T cells than APC-specific plasmid-immunized mice. In addition, the in vivo proliferative responses elicited by a non-APC-specific plasmid administration were dependent on TA-P, but were independent of CD4(+) T cell help. Collectively, our results suggest that cross-priming, in which Ags expressed in non-APCs are taken up, processed, and presented by APCs, plays an important role in the initiation, magnitude, and maintenance of CD8(+) T cell responses in gene gun DNA immunization.
- Keywords
- COLONY-STIMULATING FACTOR; EPIDERMAL LANGERHANS CELLS; MHC CLASS-I; IMMUNOSTIMULATORY DENDRITIC CELLS; ANTIGEN-PRESENTING CELLS; MARROW-DERIVED CELLS; TRANSGENIC MICE; APOPTOTIC CELLS; PLASMID DNA; EFFICIENT PRESENTATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19313
- DOI
- 10.4049/jimmunol.167.10.5549
- ISSN
- 0022-1767
- Article Type
- Article
- Citation
- JOURNAL OF IMMUNOLOGY, vol. 167, no. 10, page. 5549 - 5557, 2001-11-15
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