DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, YS | - |
dc.contributor.author | Hong, HB | - |
dc.contributor.author | Kim, BH | - |
dc.contributor.author | Hall, SC | - |
dc.contributor.author | Settineri, TA | - |
dc.date.accessioned | 2016-03-31T13:15:03Z | - |
dc.date.available | 2016-03-31T13:15:03Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2001-08-24 | - |
dc.identifier.issn | 1387-3806 | - |
dc.identifier.other | 2001-OAK-0000002187 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19405 | - |
dc.description.abstract | Protein identification can be accomplished with an enzymatic digestion of the protein followed by mass spectrometric analysis of the peptide mass fingerprint followed by database searching. However, if a protein is not in a database, sequence information must be obtained to characterize and identify it. This can be done either by classical Edman sequencing or/and by tandem mass spectrometry. To determine the sequence of an unknown protein from Bacillus pumilus PH-01, which adsorbs environmental pollutants such as polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs), both sequencing and de novo peptide sequencing by tandem MS/MS of the peptide fragments were performed. Edman sequencing of the reduced and alkylated protein revealed the majority of the sequence; however, all information on disulfide bonding was lost. Therefore, a tryptic digest of the native protein was performed to obtain both complete sequence information and the connectivity of the disulfide bonds. We performed the de novo sequencing using a hybrid quadrupole-time-of-flight mass spectrometer (Q-TOF MS) instrument. The high mass accuracy and sensitivity of the hybrid Q-TOF MS made low-level sequencing of this novel naturally isolated protein possible. (C) 2001 Elsevier Science B.V. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | - |
dc.subject | protein identification | - |
dc.subject | sequencing | - |
dc.subject | protein | - |
dc.subject | Bacillus pumilus | - |
dc.subject | hybrid Q-TOF MS | - |
dc.subject | MS/MS | - |
dc.subject | SOURCE DECAY | - |
dc.subject | PEPTIDE | - |
dc.subject | IDENTIFICATION | - |
dc.title | Sequencing of novel protein from Bacillus pumilus PH-01 using a high-resolution hybrid quadrupole-time-of-flight mass spectrometer | - |
dc.type | Article | - |
dc.contributor.college | 환경공학부 | - |
dc.identifier.doi | 10.1016/S1387-3806(01)00463-8 | - |
dc.author.google | Chang, YS | - |
dc.author.google | Hong, HB | - |
dc.author.google | Kim, BH | - |
dc.author.google | Hall, SC | - |
dc.author.google | Settineri, TA | - |
dc.relation.volume | 209 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 47 | - |
dc.relation.lastpage | 55 | - |
dc.contributor.id | 10086108 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v.209, no.1, pp.47 - 55 | - |
dc.identifier.wosid | 000170964500006 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 55 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 47 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | - |
dc.citation.volume | 209 | - |
dc.contributor.affiliatedAuthor | Chang, YS | - |
dc.identifier.scopusid | 2-s2.0-0035944247 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 5 | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | protein identification | - |
dc.subject.keywordAuthor | sequencing | - |
dc.subject.keywordAuthor | protein | - |
dc.subject.keywordAuthor | Bacillus pumilus | - |
dc.subject.keywordAuthor | hybrid Q-TOF MS | - |
dc.subject.keywordAuthor | MS/MS | - |
dc.relation.journalWebOfScienceCategory | Physics, Atomic, Molecular & Chemical | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalResearchArea | Spectroscopy | - |
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