DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hur, EM | - |
dc.contributor.author | Park, TJ | - |
dc.contributor.author | Kim, KT | - |
dc.date.accessioned | 2016-03-31T13:19:44Z | - |
dc.date.available | 2016-03-31T13:19:44Z | - |
dc.date.created | 2009-03-17 | - |
dc.date.issued | 2001-05 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.other | 2001-OAK-0000001940 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19580 | - |
dc.description.abstract | Extracellular ATP elevates cytosolic Ca2+ by activating P2X and P2Y purinoceptors and voltage-sensitive Ca2+ channels (VCCCs) in PC-12 cells, thereby facilitating catecholamine secretion. We investigated the mechanism by which ATP activates VSCCs. 2- Methylthioadenosine 5'-triphosphate (2-MeS-ATP) and UTP were used as preferential activators of P2X and P2Y, respectively. Nifedipine inhibited the ATP- and 2-MeS-ATP-evoked cytosolic Ca2+ concentration increase and [H-3] norepinephrine secretion, but not the UTP-evoked responses. Studies with Ca2+ channel blockers indicated that L-type VSCCs were activated after the P2X activation. Mn2+ entry profiles and studies with thapsigargin revealed that Ca2+ entry, rather than Ca2+ release, was sensitive to nifedipine. Although P2X(2) and P2X(4) receptor mRNAs were detected, studies with pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid revealed that P2X(2) was mainly coupled to the L-type VSCCs. The inhibitory effect of nifedipine did not occur in the absence of extracellular Na+, suggesting that Na+ influx, which induces depolarization, was essential for the P2X(2)-mediated activation of VSCCs. We report that depolarization induced by Na+ entry through the P2X(2) purinoceptors effectively activates L-type VSCCs in PC-12 cells. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.subject | ATP | - |
dc.subject | nifedipine | - |
dc.subject | depolarization | - |
dc.subject | RAT PHEOCHROMOCYTOMA CELLS | - |
dc.subject | PC12 CELLS | - |
dc.subject | ATP RECEPTOR | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | CA2+ CHANNELS | - |
dc.subject | NA+ INFLUX | - |
dc.subject | NEURONS | - |
dc.subject | ELEMENTARY | - |
dc.subject | ACTIVATION | - |
dc.subject | INHIBITION | - |
dc.title | Coupling of L-type voltage-sensitive calcium channels to P2X(2) purinoceptors in PC-12 cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1152/ajpcell.2001.280.5.C1121 | - |
dc.author.google | Hur, EM | - |
dc.author.google | Park, TJ | - |
dc.author.google | Kim, KT | - |
dc.relation.volume | 280 | - |
dc.relation.issue | 5 | - |
dc.contributor.id | 10104775 | - |
dc.relation.journal | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.280, no.5, pp.C1121 - C1129 | - |
dc.identifier.wosid | 000168221100012 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | C1129 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | C1121 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.citation.volume | 280 | - |
dc.contributor.affiliatedAuthor | Kim, KT | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 20 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RAT PHEOCHROMOCYTOMA CELLS | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | ATP RECEPTOR | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | CA2+ CHANNELS | - |
dc.subject.keywordPlus | NA+ INFLUX | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | ELEMENTARY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordAuthor | ATP | - |
dc.subject.keywordAuthor | nifedipine | - |
dc.subject.keywordAuthor | depolarization | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Physiology | - |
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