DC Field | Value | Language |
---|---|---|
dc.contributor.author | Suh, YS | - |
dc.contributor.author | Ha, SJ | - |
dc.contributor.author | Lee, CH | - |
dc.contributor.author | Sin, JI | - |
dc.contributor.author | Sung, YC | - |
dc.date.accessioned | 2016-03-31T13:20:58Z | - |
dc.date.available | 2016-03-31T13:20:58Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2001-02-28 | - |
dc.identifier.issn | 0264-410X | - |
dc.identifier.other | 2001-OAK-0000001873 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19625 | - |
dc.description.abstract | It has been reported that co-delivery of IL-12 DNA with a DNA vaccine further enhances antigen (Ag)-specific protective immunity in pathogenic challenge models. However. the enhancing effects of antibody by IL-12 have been controversial. To clarify this issue, we constructed an IL-12 expression vector, co-immunized IL-12 DNA with an encephalomycocarditis virus (EMCV)-D VPI plasmid vaccine, and then evaluated immune modulatory effects and protection against lethal EMCV-K challenge. We observed that VP1-specific IgG production, as well as seroconversion rates, were significantly enhanced by IL-12 co-injection. indicating that IL-12 can enhance antibody responses in this model system. In particular, co-injection with VPI plus IL-12 DNA into the same leg enhanced systemic Ag-specific Ige production to a significantly greater extent than either the separate leg injection of VPI and IL-12 DNA or VPI DNA vaccine alone. This suggests that local co-expression of IL-12 along with antigens is more important for enhanced antibody production. Furthermore, IgG2a isotype was significantly enhanced by IL-12 DNA co-injection. indicating a Th1 bias. In addition, co-delivery of IL-12 DNA was demonstrated to enhance VP1-specific Th cell proliferative responses. When animals were challenged with a lethal dose of EMCV-K, IL-12 DNA-co-immunized animals exhibited enhanced survival, as compared to VP1 DNA vaccine alone. These studies suggest that IL-12 plays an important role in increasing Ag-specific Th1 type antibody and cellular responses, resulting in enhanced protection against lethal EMCV-K challenge. (C) 2001 Elsevier Science Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | VACCINE | - |
dc.subject | IL-12 | - |
dc.subject | EMCV VP1 | - |
dc.subject | protection | - |
dc.subject | Th1/Th2 | - |
dc.subject | STIMULATORY FACTOR NKSF | - |
dc.subject | MOUSE SKELETAL-MUSCLE | - |
dc.subject | IN-VIVO TRANSFECTION | - |
dc.subject | ENCEPHALOMYOCARDITIS VIRUS | - |
dc.subject | DENDRITIC CELLS | - |
dc.subject | SURFACE-ANTIGEN | - |
dc.subject | MENGO-VIRUS | - |
dc.subject | FACTOR GENE | - |
dc.subject | D-VARIANT | - |
dc.subject | CYTOKINE | - |
dc.title | Enhancement of VP1-specific immune responses and protection against EMCV-K challenge by co-delivery of IL-12 DNA with VP1 DNA vaccine | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1016/S0264-410X(00)00443-6 | - |
dc.author.google | Suh, YS | - |
dc.author.google | Ha, SJ | - |
dc.author.google | Lee, CH | - |
dc.author.google | Sin, JI | - |
dc.author.google | Sung, YC | - |
dc.relation.volume | 19 | - |
dc.relation.issue | 15-16 | - |
dc.relation.startpage | 1891 | - |
dc.relation.lastpage | 1898 | - |
dc.contributor.id | 10053752 | - |
dc.relation.journal | VACCINE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | VACCINE, v.19, no.15-16, pp.1891 - 1898 | - |
dc.identifier.wosid | 000167538200007 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1898 | - |
dc.citation.number | 15-16 | - |
dc.citation.startPage | 1891 | - |
dc.citation.title | VACCINE | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | Sung, YC | - |
dc.identifier.scopusid | 2-s2.0-0035961563 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 8 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | STIMULATORY FACTOR NKSF | - |
dc.subject.keywordPlus | MOUSE SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | IN-VIVO TRANSFECTION | - |
dc.subject.keywordPlus | ENCEPHALOMYOCARDITIS VIRUS | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | SURFACE-ANTIGEN | - |
dc.subject.keywordPlus | MENGO-VIRUS | - |
dc.subject.keywordPlus | FACTOR GENE | - |
dc.subject.keywordPlus | D-VARIANT | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordAuthor | IL-12 | - |
dc.subject.keywordAuthor | EMCV VP1 | - |
dc.subject.keywordAuthor | protection | - |
dc.subject.keywordAuthor | Th1/Th2 | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
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