DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, NC | - |
dc.contributor.author | Kim, MS | - |
dc.contributor.author | Lee, WT | - |
dc.contributor.author | Choi, KY | - |
dc.contributor.author | Oh, BH | - |
dc.date.accessioned | 2016-03-31T13:23:54Z | - |
dc.date.available | 2016-03-31T13:23:54Z | - |
dc.date.created | 2009-03-17 | - |
dc.date.issued | 2000-12-29 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2001-OAK-0000001711 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19736 | - |
dc.description.abstract | Delta (5)-3-Ketosteroid isomerase catalyzes cleavage and formation of a C-H bond at a diffusion-controlled limit. By determining the crystal structures of the enzyme in complex with each of three different inhibitors and by nuclear magnetic resonance (NMR) spectroscopic investigation, we evidenced the ionization of a hydroxyl group (pK(a) similar to 16.5) of an inhibitor, which forms a low barrier hydrogen bond (LBHB) with a catalytic residue Tyr(14) (pK(a) similar to 11.5), and the protonation of the catalytic residue Asp(38) With pK(a) of similar to4.5 at pH 6.7 in the interaction with a carboxylate group of an inhibitor. The perturbation of the pK(a) values in both cases arises from the formation of favorable interactions between inhibitors and catalytic residues. The results indicate that the pK(a) difference between catalytic residue and substrate can be significantly:reduced in the active site environment as:a result of the formation of energetically favorable interactions during the course of enzyme reactions. The reduction in the pK(a) difference should facilitate the abstraction of a proton and thereby eliminate a large fraction of activation energy in general acid/base enzyme reactions. The pK(a) perturbation provides a mechanistic ground for the fast reactivity of many enzymes and for the understanding of how some enzymes are able to extract a proton from a C-H group with a pK(a) value as high as similar to 30. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | BARRIER HYDROGEN-BONDS | - |
dc.subject | DELTA(5)-3-KETOSTEROID ISOMERASE | - |
dc.subject | DELTA-5-3-KETOSTEROID ISOMERASE | - |
dc.subject | CARBON ACIDS | - |
dc.subject | 3-OXO-DELTA(5)-STEROID ISOMERASE | - |
dc.subject | PSEUDOMONAS-TESTOSTERONI | - |
dc.subject | ELECTROPHILIC CATALYSIS | - |
dc.subject | SPECTROSCOPIC EVIDENCE | - |
dc.subject | FRACTIONATION FACTORS | - |
dc.subject | DIRECTED MUTAGENESIS | - |
dc.title | Detection of large pK(alpha) perturbations of an inhibitor and a catalytic group at an enzyme active site, a mechanistic basis for catalytic power of many enzymes | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/jbc.M007561200 | - |
dc.author.google | Ha, NC | - |
dc.author.google | Kim, MS | - |
dc.author.google | Lee, WT | - |
dc.author.google | Choi, KY | - |
dc.author.google | Oh, BH | - |
dc.relation.volume | 275 | - |
dc.relation.issue | 52 | - |
dc.relation.startpage | 41100 | - |
dc.relation.lastpage | 41106 | - |
dc.contributor.id | 10052985 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.275, no.52, pp.41100 - 41106 | - |
dc.identifier.wosid | 000166114600060 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 41106 | - |
dc.citation.number | 52 | - |
dc.citation.startPage | 41100 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 275 | - |
dc.contributor.affiliatedAuthor | Kim, MS | - |
dc.contributor.affiliatedAuthor | Choi, KY | - |
dc.contributor.affiliatedAuthor | Oh, BH | - |
dc.identifier.scopusid | 2-s2.0-0034731147 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 44 | - |
dc.description.scptc | 45 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BARRIER HYDROGEN-BONDS | - |
dc.subject.keywordPlus | DELTA(5)-3-KETOSTEROID ISOMERASE | - |
dc.subject.keywordPlus | DELTA-5-3-KETOSTEROID ISOMERASE | - |
dc.subject.keywordPlus | CARBON ACIDS | - |
dc.subject.keywordPlus | 3-OXO-DELTA(5)-STEROID ISOMERASE | - |
dc.subject.keywordPlus | PSEUDOMONAS-TESTOSTERONI | - |
dc.subject.keywordPlus | ELECTROPHILIC CATALYSIS | - |
dc.subject.keywordPlus | SPECTROSCOPIC EVIDENCE | - |
dc.subject.keywordPlus | FRACTIONATION FACTORS | - |
dc.subject.keywordPlus | DIRECTED MUTAGENESIS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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