DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Hahm, B | - |
dc.contributor.author | Kim, YK | - |
dc.contributor.author | Choi, MY | - |
dc.contributor.author | Jang, SK | - |
dc.date.accessioned | 2016-03-31T13:31:14Z | - |
dc.date.available | 2016-03-31T13:31:14Z | - |
dc.date.created | 2009-08-19 | - |
dc.date.issued | 2000-05-05 | - |
dc.identifier.issn | 0022-2836 | - |
dc.identifier.other | 2000-OAK-0000001314 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/20010 | - |
dc.description.abstract | Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in several RNA-related biological processes such as transcription, pre-mRNA processing, mature mRNA transport to the cytoplasm, and translation. About 20 major hnRNPs from A1 to U are known. Among them, hnRNP A, D, E, I, and K are known to shuttle between the nucleus and the cytoplasm. hnRNP E2 has been seen to stabilize alpha-globin mRNA and to enhance polioviral mRNA translation. hnRNP K modulates transcription and translation of some mRNAs. hnRNP I and its homologue hnRNP L have been suggested to enhance translation of some IRES-dependent mRNAs. In order to better understand the molecular mechanisms of the biological functions of hnRNPs, we investigated protein-protein interactions of six hnRNPs (hnRNP Al, C1, E2, I, K, and L) using the yeast two-hybrid system and in vitro co-precipitation assays. All of the hnRNPs tested exerted homomeric interactions, and hnRNP E2, I, K, and L interacted with each other. In the case of hnRNP E2 and hnRNP K, the N-terminal half of the proteins containing two KH (K homologous) domains were required for protein-protein interaction, and the second quarter of hnRNP I and hnRNP L containing RRM2 (RNA recognition motif 2) was essential for protein-protein interaction. hnRNP A1 and C1 did not form complexes with other hnRNPs in our assay systems. This suggests that the hnRNPs could fall into two groups: one group, including hnRNP A1 and C1, involved in hnRNP core complex formation and another group, including hnRNP E2, I, K, and L, involved in a variety of RNA-related biological processes. Different combinations of the proteins of the second group may facilitate different biological processes in conjunction with other factors. (C) 2000 Academic Press. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS LTD | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR BIOLOGY | - |
dc.subject | shuttling hnRNPs | - |
dc.subject | protein-protein interaction | - |
dc.subject | yeast two-hybrid | - |
dc.subject | in vitro co-precipitation | - |
dc.subject | RNA-related processes | - |
dc.subject | TRACT-BINDING-PROTEIN | - |
dc.subject | MESSENGER-RNA STABILITY | - |
dc.subject | INTERNAL RIBOSOMAL ENTRY | - |
dc.subject | RIBONUCLEOPROTEIN-K | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | 5&apos | - |
dc.subject | -NONCODING REGION | - |
dc.subject | POLIOVIRUS RNA | - |
dc.subject | C-PROTEINS | - |
dc.subject | VIRUS-RNA | - |
dc.subject | DOMAIN | - |
dc.title | Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1006/jmbi.2000.3687 | - |
dc.author.google | Kim, JH | - |
dc.author.google | Hahm, B | - |
dc.author.google | Kim, YK | - |
dc.author.google | Choi, MY | - |
dc.author.google | Jang, SK | - |
dc.relation.volume | 298 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 395 | - |
dc.relation.lastpage | 405 | - |
dc.contributor.id | 10088382 | - |
dc.relation.journal | JOURNAL OF MOLECULAR BIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR BIOLOGY, v.298, no.3, pp.395 - 405 | - |
dc.identifier.wosid | 000086985100005 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 405 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 395 | - |
dc.citation.title | JOURNAL OF MOLECULAR BIOLOGY | - |
dc.citation.volume | 298 | - |
dc.contributor.affiliatedAuthor | Jang, SK | - |
dc.identifier.scopusid | 2-s2.0-0034607554 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 153 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRACT-BINDING-PROTEIN | - |
dc.subject.keywordPlus | MESSENGER-RNA STABILITY | - |
dc.subject.keywordPlus | INTERNAL RIBOSOMAL ENTRY | - |
dc.subject.keywordPlus | RIBONUCLEOPROTEIN-K | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | 5&apos | - |
dc.subject.keywordPlus | -NONCODING REGION | - |
dc.subject.keywordPlus | POLIOVIRUS RNA | - |
dc.subject.keywordPlus | C-PROTEINS | - |
dc.subject.keywordPlus | VIRUS-RNA | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordAuthor | shuttling hnRNPs | - |
dc.subject.keywordAuthor | protein-protein interaction | - |
dc.subject.keywordAuthor | yeast two-hybrid | - |
dc.subject.keywordAuthor | in vitro co-precipitation | - |
dc.subject.keywordAuthor | RNA-related processes | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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