Enhanced cellular immunity to hepatitis C virus nonstructural proteins by codelivery of granulocyte macrophage-colony stimulating factor gene in intramuscular DNA immunization
SCIE
SCOPUS
- Title
- Enhanced cellular immunity to hepatitis C virus nonstructural proteins by codelivery of granulocyte macrophage-colony stimulating factor gene in intramuscular DNA immunization
- Authors
- Cho, JH; Lee, SW; Sung, YC
- Date Issued
- 1999-03-05
- Publisher
- ELSEVIER SCI LTD
- Abstract
- Hepatitis C virus (HCV) nonstructural (NS) proteins appeared to be important targets for HCV vaccine development, since NS-specific T-helper-cell responses are associated with clearance from acute HCV infection. In this report, we have constructed a plasmid, pTV-NS345, that encodes the HCV NS3, NS4 and NS5 proteins (NS345) and a bicistronic plasmid, PIV-NS345/GMCSF, in which the HCV NS345 polyprotein and GMCSF are translated independently. Intramuscular inoculation with pTV-NS345 plasmid DNA into the Buffalo rats generated both antibody and T-cell proliferative responses to each NS protein. The expression of GMCSF, together with HCV NS345 proteins, appeared to significantly increase T-cell proliferative responses. In particular, the inoculation of a bicistronic plasmid generated higher T-cell proliferative responses to each NS protein than did the coinjection of two separate plasmids, pTV-NS345 and pTV-GMCSF. These results demonstrate that the codelivery of GMCSF augmented HCV NS345-specific cellular immunity and that the intensity of the immunity was differed depending on how GMCSF gene is codelivered. (C) 1999 Elsevier Science Ltd. All rights reserved.
- Keywords
- hepatitis C virus; nonstructural protein; DNA immunization; bicistronic plasmid; T-LYMPHOCYTE RESPONSE; PLASMID DNA; PROTECTIVE IMMUNITY; EXPRESSION VECTOR; DIRECT-INJECTION; CTL RESPONSES; NON-A; INFECTION; MICE; CELLS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20471
- DOI
- 10.1016/S0264-410X(98)00333-8
- ISSN
- 0264-410X
- Article Type
- Article
- Citation
- VACCINE, vol. 17, no. 9-10, page. 1136 - 1144, 1999-03-05
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