Polypyrimidine tract-binding protein interacts with HnRNP L
SCIE
SCOPUS
- Title
- Polypyrimidine tract-binding protein interacts with HnRNP L
- Authors
- Hahm, B; Cho, OH; Kim, JE; Kim, YK; Kim, JH; Oh, YL; Jang, SK
- Date Issued
- 1998-04-03
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L. (C) 1998 Federation of European Biochemical Societies.
- Keywords
- polypyrimidine tract-binding protein; heterogeneous nuclear ribonucleoprotein L; protein-protein interaction; ENCEPHALOMYOCARDITIS VIRUS-RNA; INTERNAL RIBOSOMAL ENTRY; MOUTH-DISEASE VIRUS; TRANSLATION INITIATION; SITE; INVOLVEMENT; COMPONENT; REGION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20817
- DOI
- 10.1016/S0014-5793(98)00269-5
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS LETTERS, vol. 425, no. 3, page. 401 - 406, 1998-04-03
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- There are no files associated with this item.
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