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Enhanced detoxification of organophosphates using recombinant Escherichia coli with co-expression of organophosphorus hydrolase and bacterial hemoglobin SCIE SCOPUS
- Title
- Enhanced detoxification of organophosphates using recombinant Escherichia coli with co-expression of organophosphorus hydrolase and bacterial hemoglobin
- Date Issued
- 2002-06
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- A significantly improved, recombinant Escherichia coli has been developed to degrade the toxic organophosphorus compound, Paraoxon, to non-toxic materials by co-expression of organophosphorus hydrolase (OPH) under trc promoter and Vitreoscilla hemoglobin (VHb) under O-2 dependent nar promoter. VHb-expressing whole cells had significant enhancement of OPH activity (48%, 18.7 vs. 27.8 unit l(-1)) and bioconversion efficiency V-max/K-m (44%, 0.14 vs. 0.2 min(-1)) compared to VHb-free system.
- Keywords
- bacterial hemoglobin; organophosphate; organophosphorus hydrolase; recombinant Escherichia coli; whole cell bioconversion; PSEUDOMONAS-DIMINUTA; NUCLEOTIDE-SEQUENCE; GENE; PHOSPHOTRIESTERASE; HYDROLYSIS; PROMOTER; CLONING; DEGRADATION; PROTEIN; FUSION
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 24, no. 11, page. 879 - 883, 2002-06
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Related Researcher
- 차형준CHA, HYUNG JOON
- Dept. of Chemical Enginrg