DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sin, JI | - |
dc.contributor.author | Sung, JH | - |
dc.contributor.author | Suh, YS | - |
dc.contributor.author | Lee, AH | - |
dc.contributor.author | Chung, JH | - |
dc.contributor.author | Sung, YC | - |
dc.date.accessioned | 2016-03-31T14:06:23Z | - |
dc.date.available | 2016-03-31T14:06:23Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 1997-12 | - |
dc.identifier.issn | 0264-410X | - |
dc.identifier.other | 1997-OAK-0000009996 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21159 | - |
dc.description.abstract | For DNA vaccination studies, recombinant VP1 protein of encephalomyocarditis virus (EMCV) was produced from Escherichia coli, and eukaryotic VP1 expression vector pCT-Gs-VP1, was generated and used as a DNA vaccine, Mice were immunized intramuscularly (i.m.) with pCT-Gs-VP1 in the presence or absence of plasmid DNA expressing granulocyte-macrophage colony stimulating factor (GM-CSF), and were subsequently analyzed for their anti-VP1 immune responses with recombinant VPI in ELISA, Immunization of mice with pCT-Gs-VP1 resulted in I ipl-specific immune response and 43% protection from subsequent lethal heterologous challenge of EMCV. Coinjection of mice with pCT-Gs-VP1 and plasmid DNA encoding GM-CSF was shown to increase the seroconversion rate of the immunized mice with a single DNA injection, and enhanced to a higher degree VP1-specific immunity, which appeared to result in better protection (about 80%) from lethal virus challenge. Thus, our results provide evidence for the potential use of CM-CSF to induce better immune response and resistance against viral infection in DNA vaccination. (C) 1997 Elsevier Science Ltd. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | VACCINE | - |
dc.subject | DNA vaccination | - |
dc.subject | EMCV | - |
dc.subject | GM-CSF | - |
dc.subject | SKELETAL-MUSCLE | - |
dc.subject | PLASMID DNA | - |
dc.subject | D-VARIANT | - |
dc.subject | RESPONSES | - |
dc.subject | MICE | - |
dc.subject | IMMUNIZATION | - |
dc.subject | PRODUCTS | - |
dc.subject | PROTEIN | - |
dc.subject | ANTIGEN | - |
dc.subject | INVIVO | - |
dc.title | Protective immunity against heterologous challenge with encephalomyocarditis virus by VP1 DNA vaccination: effect of coinjection with a granulocyte-macrophage colony stimulating factor gene | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1016/S0264-410X(97)88856-1 | - |
dc.author.google | SIN, JI | - |
dc.author.google | SUNG, JH | - |
dc.author.google | SUH, YS | - |
dc.author.google | LEE, AH | - |
dc.author.google | CHUNG, JH | - |
dc.author.google | SUNG, YC | - |
dc.relation.volume | 15 | - |
dc.relation.issue | 17-18 | - |
dc.relation.startpage | 1827 | - |
dc.relation.lastpage | 1833 | - |
dc.contributor.id | 10053752 | - |
dc.relation.journal | VACCINE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | VACCINE, v.15, no.17-18, pp.1827 - 1833 | - |
dc.identifier.wosid | A1997YJ42800004 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 1833 | - |
dc.citation.number | 17-18 | - |
dc.citation.startPage | 1827 | - |
dc.citation.title | VACCINE | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Sung, YC | - |
dc.identifier.scopusid | 2-s2.0-0030735550 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 67 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | D-VARIANT | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | PRODUCTS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | INVIVO | - |
dc.subject.keywordAuthor | DNA vaccination | - |
dc.subject.keywordAuthor | EMCV | - |
dc.subject.keywordAuthor | GM-CSF | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
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