DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, KJ | - |
dc.contributor.author | Suh, YA | - |
dc.contributor.author | Cho, YG | - |
dc.contributor.author | Cho, YS | - |
dc.contributor.author | Ha, GW | - |
dc.contributor.author | Chung, KH | - |
dc.contributor.author | Hwang, JH | - |
dc.contributor.author | Yun, YD | - |
dc.contributor.author | Lee, DS | - |
dc.contributor.author | Kim, CM | - |
dc.contributor.author | Sung, YC | - |
dc.date.accessioned | 2016-03-31T14:06:29Z | - |
dc.date.available | 2016-03-31T14:06:29Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 1997-11-28 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 1997-OAK-0000009992 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21162 | - |
dc.description.abstract | The envelope protein of hepatitis C virus (HCV) is composed of two membrane-associated glycoproteins, E1 and E2. To obtain HCV E2 protein as a secretory form at a high level, we constructed a recombinant chinese hamster ovary (CHO) cell line expressing a C-terminal truncated E2 (E2t) fused to human growth hormone (hGH), CHO/hGHE2t. The hGHE2t fusion protein was purified from the culture supernatant using anti-hGH mAb affinity chromatography at approximately 80% purity. The purified hGHE2t protein appeared to be assembled into oligomers linked by intermolecular disulfide bond(s) when density gradient centrifugation and SDS-polyacrylamide gel electrophoresis were employed. When the purified fusion protein was used for testing its ability to bind to antibodies specific for HCV by enzyme-linked immunosorbent assay, the protein was recognized by antibodies in sera from 90% of HCV-positive patients. Treatment of hGHE2t protein by beta-mercapto-ethanol, but not by heat and SDS, significantly reduced its reactivity to the antibodies of patient sera, suggesting that intermolecular and/or intramolecular disulfide bonds are important for its ability to recognize its specific antibody and that the E2 protein contains discontinuous antigenic epitope(s). | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | INSECT CELLS | - |
dc.subject | NEUTRALIZING ANTIBODIES | - |
dc.subject | ENVELOPE GLYCOPROTEINS | - |
dc.subject | EXPRESSED PROTEIN | - |
dc.subject | NON-A | - |
dc.subject | GENOME | - |
dc.subject | PURIFICATION | - |
dc.subject | CLEAVAGE | - |
dc.subject | E1 | - |
dc.subject | OLIGOMERIZATION | - |
dc.title | Hepatitis C virus E2 protein purified from mammalian cells is frequently recognized by E2-specific antibodies in patient sera | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/jbc.272.48.30040 | - |
dc.author.google | LEE, KJ | - |
dc.author.google | SUH, YA | - |
dc.author.google | CHO, YG | - |
dc.author.google | CHO, YS | - |
dc.author.google | HA, GW | - |
dc.author.google | CHUNG, KH | - |
dc.author.google | HWANG, JH | - |
dc.author.google | YUN, YD | - |
dc.author.google | LEE, DS | - |
dc.author.google | KIM, CM | - |
dc.author.google | SUNG, YC | - |
dc.relation.volume | 272 | - |
dc.relation.issue | 48 | - |
dc.relation.startpage | 30040 | - |
dc.relation.lastpage | 30046 | - |
dc.contributor.id | 10053752 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.272, no.48, pp.30040 - 30046 | - |
dc.identifier.wosid | A1997YH61300011 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 30046 | - |
dc.citation.number | 48 | - |
dc.citation.startPage | 30040 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 272 | - |
dc.contributor.affiliatedAuthor | Sung, YC | - |
dc.identifier.scopusid | 2-s2.0-0002949185 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 19 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INSECT CELLS | - |
dc.subject.keywordPlus | NEUTRALIZING ANTIBODIES | - |
dc.subject.keywordPlus | ENVELOPE GLYCOPROTEINS | - |
dc.subject.keywordPlus | EXPRESSED PROTEIN | - |
dc.subject.keywordPlus | NON-A | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | E1 | - |
dc.subject.keywordPlus | OLIGOMERIZATION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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