Pollen of a male-sterile mutant of Arabidopsis thaliana isolated from a T-DNA insertion pool is not effectively released from the anther locule
SCIE
SCOPUS
- Title
- Pollen of a male-sterile mutant of Arabidopsis thaliana isolated from a T-DNA insertion pool is not effectively released from the anther locule
- Authors
- Park, SK; Yoon, YH; Kim, BC; Hwang, YH; Chung, IK; Nam, HG; Kim, DU
- Date Issued
- 1996-07
- Publisher
- JAPANESE SOC PLANT PHYSIOLOGISTS
- Abstract
- We have isolated a male-sterile mutant from a pool of T-DNA insertional lines of Arabidopsis thaliana generated by an in planta transformation procedure [Chang et al. (1994) Plant J. 5: 551]. Pollen in this mutant is not effectively released from anther locules after cleavage of the stomium. Most mutant pollen grains are round, in contrast to the tricolpate wild-type pollen, and some pollen grains show an abnormal surface structure. Manually released mutant pollen grains are not fertile and show defects in pollen tube germination in vitro. Genetic analysis disclosed that this lesion is due to a single recessive nuclear mutation located on chromosome 3 closely linked to the gl1 locus. The mutation locus is tightly linked to the inserted T-DNA.
- Keywords
- Arabidopsis thaliana; in planta transformation; male-sterile mutant; porl-1 mutation; T-DNA insertional mutagenesis; AGROBACTERIUM-TUMEFACIENS; MEDIATED TRANSFORMATION; GENETIC-TRANSFORMATION; MALE-FERTILITY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21524
- DOI
- 10.1093/oxfordjournals.pcp.a028984
- ISSN
- 0032-0781
- Article Type
- Article
- Citation
- PLANT AND CELL PHYSIOLOGY, vol. 37, no. 5, page. 580 - 585, 1996-07
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- There are no files associated with this item.
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