DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, JS | - |
dc.contributor.author | Min, DS | - |
dc.contributor.author | Bae, SS | - |
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Lee, YH | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, PG | - |
dc.date.accessioned | 2016-03-31T14:20:08Z | - |
dc.date.available | 2016-03-31T14:20:08Z | - |
dc.date.created | 2009-08-12 | - |
dc.date.issued | 1996-06-30 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 1996-OAK-0000009456 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21547 | - |
dc.description.abstract | Src homology (SH) 2 and 3 domains are known to be binding motifs for protein-protein interaction in signaling molecules. Among several PLC isozymes, only PLC-gamma contains the SH domain between the X and Y domains, which are known to have catalytic activity. To elucidate the functional roles of the SH2-SH2-SH3 domain of PLC-gamma 1 in cellular signaling, we constructed a truncated cDNA encoding the SH2-SH2-SH3 domain of PLC-gamma 1 (p60(SH2/SH3)) and expressed it in NIH 3T3 cells. Cells expressing p60(SH2/SH3) did not show any change in cell shape no oncogenesity. Even though in a serum depleted condition, a portion of p60(SH2/SH3) existed as constitutively phosphorylated on its tyrosine residues. Furthermore, cells expressing p60(SH2/SH3) did not respond to PDGF-induced IPs formation whereas vector-transfected control cells showed dose-dependent IPs generation upon PDGF stimulation. The tyrosine phosphorylation level of endogenous PLC-gamma 1 by PDGF, however, was comparable to that of the control cells. On the other hand, IPs accumulation by PLC-beta activation occurred to a comparable level. Taken together, p60(SH2/SH3) molecules selectively inhibited the IPs accumulation catalyzed by PLC-gamma 1. This result suggests that the SH2-SH2-SH3 domain is essential for PLC-gamma 1-mediated cellular signaling, including its own catalytic activity by protein-protein interaction. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | TYROSINE PHOSPHORYLATION | - |
dc.subject | C-GAMMA | - |
dc.subject | BOVINE BRAIN | - |
dc.subject | PROTEIN | - |
dc.subject | HOMOLOGY | - |
dc.subject | REGION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | ONCOGENE | - |
dc.title | Overexpression of SH2-SH2-SH3 domain of phospholipase C-gamma 1 blocks PDGF-induced inositol phosphate generation in NIH 3T3 cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | Chang, JS | - |
dc.author.google | Min, DS | - |
dc.author.google | Bae, SS | - |
dc.author.google | Kim, JH | - |
dc.author.google | Lee, YH | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Suh, PG | - |
dc.relation.volume | 6 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 259 | - |
dc.relation.lastpage | 265 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.6, no.3, pp.259 - 265 | - |
dc.identifier.wosid | A1996UU76200005 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 265 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 259 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.identifier.scopusid | 2-s2.0-13544261547 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | C-GAMMA | - |
dc.subject.keywordPlus | BOVINE BRAIN | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | HOMOLOGY | - |
dc.subject.keywordPlus | REGION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ONCOGENE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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