Postelectrophoresis capillary scanning method for DNA sequencing
SCIE
SCOPUS
- Title
- Postelectrophoresis capillary scanning method for DNA sequencing
- Authors
- Kim, S; Yoo, HJ; Hahn, JH
- Date Issued
- 1996-03-01
- Publisher
- AMER CHEMICAL SOC
- Abstract
- A postelectrophoresis capillary scanning method for increasing the throughput of DNA sequencing has been developed, The method features a spatially and temporally separated arrangement between capillary gel electrophoresis separation of DNA sequencing fragments and the visualization of the separation pattern, Fluorescently labeled DNA sequencing fragments of pBluescript SK(-) were produced in enzymatic chain-termination reactions and separated by capillary gel electrophoresis using a 110 cm fused silica capillary with a transparent polymer coating. A partial sequence from the DNA was then determined by repeated scanning of the capillary at a rate of 8.39 cm/s, with changing excitation wavelengths and spectral filter sets.
- Keywords
- GEL-ELECTROPHORESIS; SEPARATION; OLIGONUCLEOTIDES; FLUORESCENCE; RESOLUTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21628
- DOI
- 10.1021/ac9508107
- ISSN
- 0003-2700
- Article Type
- Article
- Citation
- ANALYTICAL CHEMISTRY, vol. 68, no. 5, page. 936 - 939, 1996-03-01
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- There are no files associated with this item.
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