PHENOTYPIC ALTERATIONS OF PETAL AND SEPAL BY ECTOPIC EXPRESSION OF A RICE MADS BOX GENE IN TOBACCO
SCIE
SCOPUS
- Title
- PHENOTYPIC ALTERATIONS OF PETAL AND SEPAL BY ECTOPIC EXPRESSION OF A RICE MADS BOX GENE IN TOBACCO
- Authors
- KANG, HG; NOH, YS; CHUNG, YY; COSTA, MA; AN, KS; AN, GH
- Date Issued
- 1995-10
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- Floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS3, which encodes a MADS-domain containing protein. The OsMADS3 amino acid sequence shows over 60% identity to AG of Arabidopsis, PLE of Antirrhinum majus, and AG/PLE homologues of petunia, tobacco, tomato, Brassica napus, and maize. Homology in the MADS box region is most conserved. RNA blot analysis indicated that the rice MADS gene was preferentially expressed in reproductive organs, especially in stamen and carpel. In situ localization studies showed that the transcript was present primarily in stamen and carpel. The function of the rice OsMADS3 was elucidated by ectopic expression of the gene under the control of the CaMV 35S promoter in a heterologous tobacco plant system. Transgenic plants exhibited an altered morphology and coloration of the perianth organs. Sepals were pale green and elongated. Limbs of the corolla were split into sections which in some plants became antheroid structures attached to tubes that resembled filaments. The phenotypes mimic the results of ectopic expression of dicot AG gene or AG homologues. These results indicate that the OsMADS3 gene is possibly an AG homologue and that the AG genes appear to be structurally and functionally conserved between dicot and monocot.
- Keywords
- AGAMOUS; FLOWER DEVELOPMENT; HOMEOTIC GENE; MADS BOX; RICE; TOBACCO; HOMEOTIC GENE; MOLECULAR CHARACTERIZATION; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; ANTIRRHINUM-MAJUS; DEFICIENS; PERIANTH; IDENTITY; PLANTS; ORGANS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21709
- DOI
- 10.1007/BF00019114
- ISSN
- 0167-4412
- Article Type
- Article
- Citation
- PLANT MOLECULAR BIOLOGY, vol. 29, no. 1, page. 1 - 10, 1995-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.