DC Field | Value | Language |
---|---|---|
dc.contributor.author | SHIN, YS | - |
dc.contributor.author | CHOI, G | - |
dc.contributor.author | HOI, KY | - |
dc.date.accessioned | 2016-03-31T14:27:17Z | - |
dc.date.available | 2016-03-31T14:27:17Z | - |
dc.date.created | 2009-03-17 | - |
dc.date.issued | 1995-08-31 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 1995-OAK-0000009213 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21731 | - |
dc.description.abstract | The entire gene encoding D-myo-inositol 1,4,5-trisphosphate 3-kinase (IP3K) of rat brain was overexpressed using a T7 promoter in an expression vector pT7-7. When induced by isopropyl-beta-D-thiogalactoside, E. coli BL21(DE3) transformed with pT7-7/IP3K expressed IP3K over 130 fold compared with rat brain. IP3K was purified to homogeneity by a simple and rapid purification procedure utilizing a calmodulin-agarose column as a major chromatographic step. The purified IP3K exhibited a polypeptide (M(r) = 50000) on sodium dodecylsulfate-polyacrylamide gel electrophoresis which shows specific cross-reactivity towards monoclonal antibodies raised against IP3K from rat brain. The purified IP3K has a specific activity of 20 mu mole/min/mg protein in the absence of calmodulin, with 1.5 fold activation of the activity by Ca2+/calmodulin. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | EXPRESSION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | CALMODULIN | - |
dc.subject | KINASE | - |
dc.subject | SITE | - |
dc.title | OVEREXPRESSION, PURIFICATION AND CHARACTERIZATION OF INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE FROM RAT-BRAIN | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | SHIN, YS | - |
dc.author.google | CHOI, G | - |
dc.author.google | HOI, KY | - |
dc.relation.volume | 5 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 348 | - |
dc.relation.lastpage | 353 | - |
dc.contributor.id | 10052985 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.5, no.4, pp.348 - 353 | - |
dc.identifier.wosid | A1995RQ85500009 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 353 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 348 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | HOI, KY | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CALMODULIN | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | SITE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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