TAT AND NEF DEFECTIVE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATES EFFICIENTLY IN TAT-EXPRESSING T-CELLS
SCIE
- Title
- TAT AND NEF DEFECTIVE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATES EFFICIENTLY IN TAT-EXPRESSING T-CELLS
- Authors
- LEE, AH; SUH, YA; SUNG, YC
- Date Issued
- 1995-08-31
- Publisher
- KOREAN SOC MOLECULAR BIOLOGY
- Abstract
- Human immunodeficiency virus type 1 (HIV-1) contains several nonstructural genes which are required for viral replication and disease pathogenesis. Among them, rat and nef genes encode an essential transactivator of HIV-1 LTR and a pluripotent protein which seems to be essential for in vivo but not in vitro viral replication, respectively. We constructed two tat and nef defective HIV-1 and tested for their abilities Co replicate in several T cells. The defective viruses did not replicate in CD4(+) T cells, but were rescued in the recombinant Jurkat-tat cells which express the rat gene. The replication of HXB/tnC which is defective in far and nef, and contains the chloramphenicol acetyltransferase (CAT) gene was easily detected by a CAT assay. No revertant was identified during the passages of the mutant-viruses for more than two months in Jurkat-tat cells, far and nef defective HIV-1 could be used instead of wild type viruses for several purposes such as inhibitor screening and development of killed AIDS vaccine.
- Keywords
- TRANS-ACTIVATOR GENE; LONG TERMINAL REPEAT; CHLORAMPHENICOL ACETYLTRANSFERASE; DOWN-REGULATION; HTLV-III; HIV-1; PROTEIN; CONSTRUCTION; ELEMENTS; ALLELES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21733
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 5, no. 4, page. 306 - 310, 1995-08-31
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