DC Field | Value | Language |
---|---|---|
dc.contributor.author | SUH, BC | - |
dc.contributor.author | KIM, KT | - |
dc.date.accessioned | 2016-03-31T14:29:51Z | - |
dc.date.available | 2016-03-31T14:29:51Z | - |
dc.date.created | 2009-03-18 | - |
dc.date.issued | 1995-06 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.other | 1995-OAK-0000009141 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21787 | - |
dc.description.abstract | Muscarinic receptor in human neuroblastoma SK-N-BE(2)C cells was identified and characterized. Treatment of the cells with carbachol evoked the generation of inositol 1,4,5-trisphosphate (IP3) with a peak level reached at 1 min after stimulation. Carbachol increased intracellular Ca2+ ([Ca2+](i)) with an EC(50) value of 35 mu M. In addition, carbachol produced a 1.3-3-fold increase in the cyclic AMP (cAMP) level compared with untreated control and elevated synergistically the cAMP level in the treatment with prostaglandin E(2) (PGE(2)). The M(3) antagonist p-fluorohexahydrosiladifenidol (IC50 = 0.5-0.8 mu M) inhibited the increases in [Ca2+](i), IP3, and cAMP more effectively than the M(1) antagonist pirenzepine (IC50 = 5-9 mu M) and the M(2) antagonist methoctramine (IC50 = 20-30 mu M) The involvements of [Ca2+](i) elevation and protein kinase C activation induced by phospholipase C activation were tested in the carbachol-induced cAMP production. The calcium chelator BAPTA/AM (75 mu M) inhibited significantly the synergistic effects of carbachol and PGE(2) on the production of cAMP, whereas the Ca2+ ionophore ionomycin (1 mu M) clearly enhanced PGE(2)-induced cAMP production. However, phorbol 12-myristate 13-acetate did not enhance PGE(2)-stimulated cAMP production. These data suggest that phospholipase C-linked M(3) receptors are present and that stimulation of the receptors activates adenylyl cyclase, at least in part, by the Ca2+-dependent system in the neuronal cells. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | LIPPINCOTT-RAVEN PUBL | - |
dc.relation.isPartOf | JOURNAL OF NEUROCHEMISTRY | - |
dc.subject | SK-N-BE(2)C HUMAN NEUROBLASTOMA | - |
dc.subject | MUSCARINIC M(3) RECEPTORS | - |
dc.subject | CA2+ MOBILIZATION | - |
dc.subject | CYCLIC AMP ACCUMULATION | - |
dc.subject | INOSITOL 1,4,5-TRISPHOSPHATE | - |
dc.subject | PROTEIN-KINASE-C | - |
dc.subject | SK-N-SH | - |
dc.subject | INOSITOL 1,4,5-TRISPHOSPHATE BINDING | - |
dc.subject | NEURO-BLASTOMA CELLS | - |
dc.subject | BETA-GAMMA-SUBUNITS | - |
dc.subject | CYCLIC-AMP | - |
dc.subject | PHOSPHOINOSITIDE HYDROLYSIS | - |
dc.subject | MOLECULAR-BASIS | - |
dc.subject | PHORBOL ESTERS | - |
dc.subject | SH-SY5Y | - |
dc.title | STIMULATION OF ADENYLYL-CYCLASE MEDIATED BY PHOSPHOLIPASE C-LINKED M(3) MUSCARINIC RECEPTOR IN HUMAN NEUROBLASTOMA SK-N-BE(2)C CELLS | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1046/j.1471-4159.1995.64062500.x | - |
dc.author.google | SUH, BC | - |
dc.author.google | KIM, KT | - |
dc.relation.volume | 64 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 2500 | - |
dc.relation.lastpage | 2508 | - |
dc.contributor.id | 10104775 | - |
dc.relation.journal | JOURNAL OF NEUROCHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, v.64, no.6, pp.2500 - 2508 | - |
dc.identifier.wosid | A1995QZ25200014 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 2508 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2500 | - |
dc.citation.title | JOURNAL OF NEUROCHEMISTRY | - |
dc.citation.volume | 64 | - |
dc.contributor.affiliatedAuthor | KIM, KT | - |
dc.identifier.scopusid | 2-s2.0-0029024092 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 18 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | SK-N-SH | - |
dc.subject.keywordPlus | INOSITOL 1,4,5-TRISPHOSPHATE BINDING | - |
dc.subject.keywordPlus | NEURO-BLASTOMA CELLS | - |
dc.subject.keywordPlus | BETA-GAMMA-SUBUNITS | - |
dc.subject.keywordPlus | CYCLIC-AMP | - |
dc.subject.keywordPlus | PHOSPHOINOSITIDE HYDROLYSIS | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | PHORBOL ESTERS | - |
dc.subject.keywordPlus | SH-SY5Y | - |
dc.subject.keywordAuthor | SK-N-BE(2)C HUMAN NEUROBLASTOMA | - |
dc.subject.keywordAuthor | MUSCARINIC M(3) RECEPTORS | - |
dc.subject.keywordAuthor | CA2+ MOBILIZATION | - |
dc.subject.keywordAuthor | CYCLIC AMP ACCUMULATION | - |
dc.subject.keywordAuthor | INOSITOL 1,4,5-TRISPHOSPHATE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
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