DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, HS | - |
dc.contributor.author | LEE, YH | - |
dc.contributor.author | PAI, JK | - |
dc.contributor.author | RYU, SH | - |
dc.contributor.author | SUH, PG | - |
dc.date.accessioned | 2016-03-31T14:35:07Z | - |
dc.date.available | 2016-03-31T14:35:07Z | - |
dc.date.created | 2009-08-12 | - |
dc.date.issued | 1994-10-28 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 1994-OAK-0000008981 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21895 | - |
dc.description.abstract | The mechanism of phospholipase D (PLD) activation by platelet derived growth factor (PDGF) was examined using a NIH 3T3 fibroblast cell line (3T3-gamma 1) that stably overexpresses PLC-gamma 1 isozyme. Immunoblot analysis revealed that 3T3-gamma 1 cells contained about 10-fold more PLC-gamma 1 than a control cell line (3T3-C) transfected with expression vector lacking PLC-gamma 1 cDNA. PDGF-stimulated PLD activation was 10-fold greater in 3T3-gamma 1 cells than in 3T3-C cells, indicating that PLD activation is dependent upon the level of PLC-gamma 1. Phorbol 12-myristate 13-acetate (PMA) treatment increased PLD activity to a similar extent in both 3T3-gamma 1 cells and control cells. Pretreatment with tyrosine kinase inhibitors including staurosporine and genistein decreased PLD activity by 82.6% and 87.2%, respectively, and completely blocked tyrosine phosphorylation of PDGF receptor and PLC-gamma 1 in 3T3-gamma 1 cells stimulated with PDGF. Moreover, downregulation of protein kinase C by pretreatment of PMA caused complete inhibition of PDGF- and PMA-stimulated PLD activation. Therefore, these results suggest that PDGF-induced PLD activation may be a consequence of primary stimulation of PLC-gamma 1 and that PLD may play a role downstream hom PLC-gamma 1 in PDGF-triggered mitogenesis. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | ACTIVATION OF PHOSPHOLIPASE-D INDUCED BY PLATELET-DERIVED GROWTH-FACTOR IS DEPENDENT UPON THE LEVEL OF PHOSPHOLIPASE C-GAMMA-1 | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | KIM, HS | - |
dc.author.google | LEE, YH | - |
dc.author.google | PAI, JK | - |
dc.author.google | RYU, SH | - |
dc.author.google | SUH, PG | - |
dc.relation.volume | 269 | - |
dc.relation.startpage | 26842 | - |
dc.relation.lastpage | 26847 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.269, no.43, pp.26842 - 26847 | - |
dc.identifier.wosid | A1994PQ93100042 | - |
dc.citation.endPage | 26847 | - |
dc.citation.number | 43 | - |
dc.citation.startPage | 26842 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 269 | - |
dc.contributor.affiliatedAuthor | RYU, SH | - |
dc.contributor.affiliatedAuthor | SUH, PG | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 86 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | SMOOTH-MUSCLE CELLS | - |
dc.subject.keywordPlus | PHOSPHATIDYLCHOLINE HYDROLYSIS | - |
dc.subject.keywordPlus | SIGNAL TRANSDUCTION | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | MITOGENIC SIGNAL | - |
dc.subject.keywordPlus | DIACYLGLYCEROL FORMATION | - |
dc.subject.keywordPlus | HL-60 GRANULOCYTES | - |
dc.subject.keywordPlus | PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | NIH-3T3 CELLS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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