Open Access System for Information Sharing

Login Library

 

Article
Cited 76 time in webofscience Cited 73 time in scopus
Metadata Downloads

GENERATION OF EXPRESSED SEQUENCE TAGS OF RANDOM ROOT CDNA CLONES OF BRASSICA-NAPUS BY SINGLE-RUN PARTIAL SEQUENCING SCIE SCOPUS

Title
GENERATION OF EXPRESSED SEQUENCE TAGS OF RANDOM ROOT CDNA CLONES OF BRASSICA-NAPUS BY SINGLE-RUN PARTIAL SEQUENCING
Authors
CHO, MJKIM, YSKWAK, JMKWON, OYLEE, DSLEE, HHNAM, HGPARK, YS
Date Issued
1993-10
Publisher
AMER SOC PLANT PHYSIOLOGISTS
Abstract
Two hundred thirty-seven expressed sequence tags (ESTs) of Brassica napus were generated by single-run partial sequencing of 197 random root cDNA clones. A computer search of these root ESTs revealed that 21 ESTs show significant similarity to the protein-coding sequences in the existing data bases, including five stress- or defense-related genes and four clones related to the genes from other kingdoms. Northern blot analysis of the 10 data base-matched cDNA clones revealed that many of the clones are expressed most abundantly in root but less abundantly in other organs. However, two clones were highly root specific. The results show that generation of the root ESTs by partial sequencing of random cDNA clones along with the expression analysis is an efficient approach to isolate genes that are functional in plant root in a large scale. We also discuss the results of the examination of cDNA libraries and sequencing methods suitable for this approach.
URI
https://oasis.postech.ac.kr/handle/2014.oak/22040
DOI
10.1104/pp.103.2.359
ISSN
0032-0889
Article Type
Article
Citation
PLANT PHYSIOLOGY, vol. 103, no. 2, page. 359 - 370, 1993-10
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

남홍길NAM, HONG GIL
Div of Integrative Biosci & Biotech
Read more

Views & Downloads

Browse