DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, HR | - |
dc.contributor.author | Lee, GH | - |
dc.contributor.author | Ha, KC | - |
dc.contributor.author | Ahn, T | - |
dc.contributor.author | Moon, JY | - |
dc.contributor.author | Lee, BJ | - |
dc.contributor.author | Cho, SG | - |
dc.contributor.author | Kim, S | - |
dc.contributor.author | Seo, YR | - |
dc.contributor.author | Shin, YJ | - |
dc.contributor.author | Chae, SW | - |
dc.contributor.author | Reed, JC | - |
dc.contributor.author | Chae, HJ | - |
dc.date.accessioned | 2016-04-01T01:20:20Z | - |
dc.date.available | 2016-04-01T01:20:20Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2008-06-06 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2008-OAK-0000007812 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/22732 | - |
dc.description.abstract | In this study, Bax inhibitor-1 (BI-1) overexpression reduces the ER pool of Ca2+ released by thapsigargin. Cells overexpressing BI-1 also showed lower intracellular Ca2+ release induced by the Ca2+ ionophore ionomycin as well as agonists of ryanodine receptors and inositol trisphosphate receptors. In contrast, cells expressing carboxyl-terminal deleted BI-1 (C Delta-BI-1 cells) displayed normal intracellular Ca2+ mobilization. Basal Ca2+ release rates from the ER were higher in BI-1-overexpressing cells than in control or C Delta-BI-1 cells. We determined that the carboxyl-terminal cytosolic region of BI-1 contains a lysine-rich motif (EKDKKKEKK) resembling the pH-sensing domains of ion channels. Acidic conditions triggered more extensive Ca2+ release from ER microsomes from BI-1-overexpressing cells and BI-1-reconsituted liposomes. Acidic conditions also induced BI-1 protein oligomerization. Interestingly subjecting BI-1 overexpressing cells to acidic conditions induced more Bax recruitment to mitochondria, more cytochrome c release from mitochondria, and more cell death. These findings suggest that BI-1 increases Ca2+ leak rates from the ER through a mechanism that is dependent on pH and on the carboxyl-terminal cytosolic region of the BI-1 protein. The findings also reveal a cell death-promoting phenotype for BI-1 that is manifested under low pH conditions. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | INTRACELLULAR PH | - |
dc.subject | CALCIUM STORES | - |
dc.subject | ION-CHANNEL | - |
dc.subject | CELLS | - |
dc.subject | APOPTOSIS | - |
dc.subject | MACROPHAGES | - |
dc.subject | MECHANISMS | - |
dc.subject | EXPRESSION | - |
dc.subject | BINDING | - |
dc.subject | STRESS | - |
dc.title | Bax inhibitor-1 is a pH-dependent regulator of Ca2+ channel activity in the endoplasmic reticulum | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/jbc.M800075200 | - |
dc.author.google | Kim, HR | - |
dc.author.google | Lee, GH | - |
dc.author.google | Ha, KC | - |
dc.author.google | Ahn, T | - |
dc.author.google | Moon, JY | - |
dc.author.google | Lee, BJ | - |
dc.author.google | Cho, SG | - |
dc.author.google | Kim, S | - |
dc.author.google | Seo, YR | - |
dc.author.google | Shin, YJ | - |
dc.author.google | Chae, SW | - |
dc.author.google | Reed, JC | - |
dc.author.google | Chae, HJ | - |
dc.relation.volume | 283 | - |
dc.relation.issue | 23 | - |
dc.relation.startpage | 15946 | - |
dc.relation.lastpage | 15955 | - |
dc.contributor.id | 10136479 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.283, no.23, pp.15946 - 15955 | - |
dc.identifier.wosid | 000256332500053 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 15955 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 15946 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 283 | - |
dc.contributor.affiliatedAuthor | Kim, S | - |
dc.identifier.scopusid | 2-s2.0-47049128540 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 71 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INTRACELLULAR PH | - |
dc.subject.keywordPlus | CALCIUM STORES | - |
dc.subject.keywordPlus | ION-CHANNEL | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | STRESS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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