Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants
SCIE
SCOPUS
- Title
- Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants
- Authors
- Lim, PO; Kim, Y; Breeze, E; Koo, JC; Woo, HR; Ryu, JS; Park, DH; Beynon, J; Tabrett, A; Buchanan-Wollaston, V; Nam, HG
- Date Issued
- 2007-12
- Publisher
- BLACKWELL PUBLISHING
- Abstract
- Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.
- Keywords
- leaf senescence; post-harvest storage; AT-hook protein; chromatin architecture; activation tagging; Arabidopsis; DNA-BINDING PROTEIN; ARABIDOPSIS-THALIANA; TRANSCRIPTION FACTOR; GENE-EXPRESSION; CHROMOSOME DYNAMICS; FUSION PROTEIN; SENESCENCE; PATHWAYS; INDUCTION; STRESS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23049
- DOI
- 10.1111/j.1365-313X.2007.03317.x
- ISSN
- 0960-7412
- Article Type
- Article
- Citation
- PLANT JOURNAL, vol. 52, no. 6, page. 1140 - 1153, 2007-12
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