Triad base pairs containing fluorene unit for quencher-free SNP typing
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SCOPUS
- Title
- Triad base pairs containing fluorene unit for quencher-free SNP typing
- Authors
- Ryu, JH; Seo, YJ; Hwang, GT; Lee, JY; Kim, BH
- Date Issued
- 2007-04-23
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Abstract
- We designed an A-selective fluorescent DNA probe, U-FL, which bears a 2-ethynylfluorene moiety covalently attached to the base dU, and incorporated it into a central position in the hairpin loop. We describe photophysical studies of triad base pairs containing an U-FL DNA probe and their application to single-nucleotide polymorphism (SNP) typing using novel quencher-free molecular beacons (MBs). The drastic changes in the fluorescence properties that arise upon changing the nature of the complementary base and FBs suggest that these triad base pairs are the key elements for quencher-free SNP typing. (c) 2007 Elsevier Ltd. All rights reserved.
- Keywords
- SINGLE NUCLEOTIDE POLYMORPHISMS; FLUORIDE-SELECTIVE FLUORESCENT; PHOTOINDUCED ELECTRON-TRANSFER; NUCLEIC-ACIDS; MOLECULAR BEACONS; DNA-HAIRPINS; OLIGONUCLEOTIDE PROBES; HYBRIDIZATION PROBES; FUNCTIONAL GENOMICS; PYRIMIDINE-BASES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23260
- DOI
- 10.1016/J.TET.2006.1
- ISSN
- 0040-4020
- Article Type
- Article
- Citation
- TETRAHEDRON, vol. 63, no. 17, page. 3538 - 3547, 2007-04-23
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- There are no files associated with this item.
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