DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lam, SK | - |
dc.contributor.author | Siu, CL | - |
dc.contributor.author | Hillmer, S | - |
dc.contributor.author | Jang, S | - |
dc.contributor.author | An, GH | - |
dc.contributor.author | Robinson, DG | - |
dc.contributor.author | Jiang, LW | - |
dc.date.accessioned | 2016-04-01T01:42:35Z | - |
dc.date.available | 2016-04-01T01:42:35Z | - |
dc.date.created | 2009-08-07 | - |
dc.date.issued | 2007-01 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.other | 2007-OAK-0000006647 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/23530 | - |
dc.description.abstract | We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to the lytic vacuole in tobacco ( Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral membrane proteins mediating endocytosis in animal cells. To define the endocytic pathway in plants, we cloned the rice ( Oryza sativa) homolog of animal SCAMP1 and generated transgenic tobacco BY-2 cells expressing yellow fluorescent protein (YFP)- SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that YFP-SCAMP1 fusions and native SCAMP1 localize to the plasma membrane and mobile structures in the cytoplasm of transgenic BY-2 cells. Drug treatments and confocal immunofluorescence studies demonstrated that the punctate cytosolic organelles labeled by YFP-SCAMP1 or SCAMP1 were distinct from the Golgi apparatus and PVCs. SCAMP1-labeled organelles may represent an early endosome because the internalized endocytic markers FM4-64 and AM4-64 reached these organelles before PVCs. In addition, wortmannin caused the redistribution of SCAMP1 from the early endosomes to PVCs, probably as a result of fusions between the two compartments. Immunogold electron microscopy with high-pressure frozen/freeze-substituted samples identified the SCAMP1-positive organelles as tubular-vesicular structures at the trans-Golgi with clathrin coats. These early endosomal compartments resemble the previously described partially coated reticulum and trans-Golgi network in plant cells. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.relation.isPartOf | PLANT CELL | - |
dc.subject | PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY | - |
dc.subject | VACUOLAR SORTING RECEPTORS | - |
dc.subject | GREEN FLUORESCENT PROTEIN | - |
dc.subject | PLANT-CELLS | - |
dc.subject | PREVACUOLAR COMPARTMENTS | - |
dc.subject | BREFELDIN-A | - |
dc.subject | MULTIVESICULAR BODIES | - |
dc.subject | SOYBEAN PROTOPLASTS | - |
dc.subject | ENDOCYTIC PATHWAY | - |
dc.subject | MEMBRANE DYNAMICS | - |
dc.title | Rice SCAMP1 defines clathrin-coated, trans-Golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1105/TPC.106.0457 | - |
dc.author.google | Lam, SK | - |
dc.author.google | Siu, CL | - |
dc.author.google | Hillmer, S | - |
dc.author.google | Jang, S | - |
dc.author.google | An, GH | - |
dc.author.google | Robinson, DG | - |
dc.author.google | Jiang, LW | - |
dc.relation.volume | 19 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 296 | - |
dc.relation.lastpage | 319 | - |
dc.contributor.id | 10184525 | - |
dc.relation.journal | PLANT CELL | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT CELL, v.19, no.1, pp.296 - 319 | - |
dc.identifier.wosid | 000244757400025 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 319 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 296 | - |
dc.citation.title | PLANT CELL | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | An, GH | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 180 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GREEN FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | BREFELDIN-A | - |
dc.subject.keywordPlus | PREVACUOLAR COMPARTMENTS | - |
dc.subject.keywordPlus | MULTIVESICULAR BODIES | - |
dc.subject.keywordPlus | PLANT-CELLS | - |
dc.subject.keywordPlus | ENDOCYTIC PATHWAY | - |
dc.subject.keywordPlus | MEMBRANE DYNAMICS | - |
dc.subject.keywordPlus | 3-KINASE ACTIVITY | - |
dc.subject.keywordPlus | SORTING SIGNALS | - |
dc.subject.keywordPlus | AUXIN TRANSPORT | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Cell Biology | - |
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