Identification of active transposon dTok, a member of the hAT family, in rice
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- Title
- Identification of active transposon dTok, a member of the hAT family, in rice
- Authors
- Moon, S; Jung, KH; Lee, DE; Jiang, WZ; Koh, HJ; Heu, MH; Lee, DS; Suh, HS; An, G
- Date Issued
- 2006-11
- Publisher
- OXFORD UNIV PRESS
- Abstract
- Recent completion of the sequencing of the rice genome has revealed that it contains > 40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5-8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar 'Nipponbare' contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.
- Keywords
- hAT superfamily; floral organ number 1/multiple pistil 2; Oryza; rice; transposon; ANTIRRHINUM-MAJUS; DNA METHYLATION; GENOME EVOLUTION; TISSUE-CULTURE; DRAFT SEQUENCE; MAIZE; ELEMENTS; ARABIDOPSIS; PLANTS; TAM3
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23676
- DOI
- 10.1093/PCP/PCL012
- ISSN
- 0032-0781
- Article Type
- Article
- Citation
- PLANT AND CELL PHYSIOLOGY, vol. 47, no. 11, page. 1473 - 1483, 2006-11
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