Real-time PCR sensing by thermal conductivity monitoring
SCIE
SCOPUS
- Title
- Real-time PCR sensing by thermal conductivity monitoring
- Authors
- Noh, S; Lee, J; Shin, JK; Lim, G
- Date Issued
- 2006-10
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- Traditional PCR (Polymerase Chain Reaction) technology is based on an end-point system which confirms the existence of a target DNA sequence by electrophoresis and UV transillumination steps. This process is labor-intensive and time consuming. A real-time PCR is the best alternative that can improve problems of analysis time and accuracy. Therefore, we propose a smaller, non-optical and non-labeling system with the advantages of an established real-time PCR. The proposed technique utilizes a variation of the thermal conductivity of the DNA sample with a PCR cycle. We confirmed that thermal conductivity depends on amounts of PCR products.
- Keywords
- real-time PCR; thermal conductivity; PCR detection method; non-labeling system; kinetic system; POLYMERASE-CHAIN-REACTION; AMPLIFICATION; QUANTIFICATION; SEQUENCES; ASSAY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/23746
- DOI
- 10.1002/elan.200603622
- ISSN
- 1040-0397
- Article Type
- Article
- Citation
- ELECTROANALYSIS, vol. 18, no. 19-20, page. 1989 - 1993, 2006-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.