Characterization of rice mutants with enhanced susceptibility to rice blast
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- Title
- Characterization of rice mutants with enhanced susceptibility to rice blast
- Authors
- Kim, HK; Lee, SK; Cho, JI; Lee, S; An, GH; Jwa, NS; Kim, BR; Cho, YC; Han, SS; Bhoo, SHF; Lee, YH; Hong, YK; Yi, GW; Park, DS; Hahn, TR; Jeon, JS
- Date Issued
- 2005-12-31
- Publisher
- SPRINGER SINGAPORE PTE LTD
- Abstract
- As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the F-2 segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the F-2 population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 F-2 plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor.
- Keywords
- blast resistance; Hwayeong; NB-LRR; rice; Pib mutation; PLANT-DISEASE RESISTANCE; ORYZA-SATIVA L.; GENOME-WIDE ANALYSIS; NUCLEOTIDE-BINDING; MAGNAPORTHE-GRISEA; DEFENSE RESPONSES; GENE PI-2(T); SSR MARKERS; ARABIDOPSIS; PATHOGEN
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24251
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 20, no. 3, page. 385 - 391, 2005-12-31
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