DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, HK | - |
dc.contributor.author | Lee, SK | - |
dc.contributor.author | Cho, JI | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | An, GH | - |
dc.contributor.author | Jwa, NS | - |
dc.contributor.author | Kim, BR | - |
dc.contributor.author | Cho, YC | - |
dc.contributor.author | Han, SS | - |
dc.contributor.author | Bhoo, SHF | - |
dc.contributor.author | Lee, YH | - |
dc.contributor.author | Hong, YK | - |
dc.contributor.author | Yi, GW | - |
dc.contributor.author | Park, DS | - |
dc.contributor.author | Hahn, TR | - |
dc.contributor.author | Jeon, JS | - |
dc.date.accessioned | 2016-04-01T02:01:44Z | - |
dc.date.available | 2016-04-01T02:01:44Z | - |
dc.date.created | 2009-08-06 | - |
dc.date.issued | 2005-12-31 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 2006-OAK-0000005603 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/24251 | - |
dc.description.abstract | As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the F-2 segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the F-2 population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 F-2 plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER SINGAPORE PTE LTD | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | blast resistance | - |
dc.subject | Hwayeong | - |
dc.subject | NB-LRR | - |
dc.subject | rice | - |
dc.subject | Pib mutation | - |
dc.subject | PLANT-DISEASE RESISTANCE | - |
dc.subject | ORYZA-SATIVA L. | - |
dc.subject | GENOME-WIDE ANALYSIS | - |
dc.subject | NUCLEOTIDE-BINDING | - |
dc.subject | MAGNAPORTHE-GRISEA | - |
dc.subject | DEFENSE RESPONSES | - |
dc.subject | GENE PI-2(T) | - |
dc.subject | SSR MARKERS | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | PATHOGEN | - |
dc.title | Characterization of rice mutants with enhanced susceptibility to rice blast | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | Kim, HK | - |
dc.author.google | Lee, SK | - |
dc.author.google | Cho, JI | - |
dc.author.google | Lee, S | - |
dc.author.google | An, GH | - |
dc.author.google | Jwa, NS | - |
dc.author.google | Kim, BR | - |
dc.author.google | Cho, YC | - |
dc.author.google | Han, SS | - |
dc.author.google | Bhoo, SHF | - |
dc.author.google | Lee, YH | - |
dc.author.google | Hong, YK | - |
dc.author.google | Yi, GW | - |
dc.author.google | Park, DS | - |
dc.author.google | Hahn, TR | - |
dc.author.google | Jeon, JS | - |
dc.relation.volume | 20 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 385 | - |
dc.relation.lastpage | 391 | - |
dc.contributor.id | 10184525 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.20, no.3, pp.385 - 391 | - |
dc.identifier.wosid | 000234426600012 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 391 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 385 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | An, GH | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PLANT-DISEASE RESISTANCE | - |
dc.subject.keywordPlus | ORYZA-SATIVA L. | - |
dc.subject.keywordPlus | GENOME-WIDE ANALYSIS | - |
dc.subject.keywordPlus | NUCLEOTIDE-BINDING | - |
dc.subject.keywordPlus | MAGNAPORTHE-GRISEA | - |
dc.subject.keywordPlus | DEFENSE RESPONSES | - |
dc.subject.keywordPlus | GENE PI-2(T) | - |
dc.subject.keywordPlus | SSR MARKERS | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | PATHOGEN | - |
dc.subject.keywordAuthor | blast resistance | - |
dc.subject.keywordAuthor | Hwayeong | - |
dc.subject.keywordAuthor | NB-LRR | - |
dc.subject.keywordAuthor | rice | - |
dc.subject.keywordAuthor | Pib mutation | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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