Mind bomb 1 is essential for generating functional Notch ligands to activate Notch
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SCOPUS
- Title
- Mind bomb 1 is essential for generating functional Notch ligands to activate Notch
- Authors
- Koo, BK; Lim, HS; Song, R; Yoon, MJ; Yoon, KJ; Moon, JS; Kim, YW; Kwon, MC; Yoo, KW; Kong, MP; Lee, J; Chitnis, AB; Kim, CH; Kong, YY
- Date Issued
- 2005-08
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Abstract
- The Delta-Notch signaling pathway is an evolutionarily conserved intercellular signaling mechanism essential for cell fate specification. Mind bomb 1 (Mib1) has been identified as a ubiquitin ligase that promotes the endocytosis of Delta. We now report that mice lacking Mib1 die prior to embryonic day 11.5, with pan-Notch defects in somitogenesis, neurogenesis, vasculogenesis and cardiogenesis. The Mib1(-/-) embryos exhibit reduced expression of Notch target genes Hes5, Hey1, Hey2 and Heyl, with the loss of N1icd generation. Interestingly, in the Mib1(-/-) mutants, DII1 accumulated in the plasma membrane, while it was localized in the cytoplasm near the nucleus in the wild types, indicating that Mib1 is essential for the endocytosis of Notch ligand. In accordance with the pan-Notch defects in Mib1(-/-) embryos, Mib1 interacts with and regulates all of the Notch ligands, jagged I and jagged 2, as well as DII1, DII3 and DII4. Our results show that Mib1 is an essential regulator, but not a potentiator, for generating functional Notch ligands to activate Notch signaling.
- Keywords
- Notch signaling; mind bomb; endocytosis; Notch ligand; mouse; DROSOPHILA-NEURALIZED GENE; UBIQUITIN LIGASE; SIGNALING PATHWAY; MICE LACKING; VASCULAR DEVELOPMENT; EMBRYONIC LETHALITY; ZEBRAFISH HINDBRAIN; PATTERNING DEFECTS; CELL FATE; DELTA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24412
- DOI
- 10.1242/DEV.01922
- ISSN
- 0950-1991
- Article Type
- Article
- Citation
- DEVELOPMENT, vol. 132, no. 15, page. 3459 - 3470, 2005-08
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