DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, MH | - |
dc.contributor.author | Lee, YA | - |
dc.contributor.author | Bae, YS | - |
dc.contributor.author | Kita, H | - |
dc.contributor.author | Kim, K | - |
dc.contributor.author | Ryu, SH | - |
dc.date.accessioned | 2016-04-01T02:09:59Z | - |
dc.date.available | 2016-04-01T02:09:59Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2005-01 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.other | 2005-OAK-0000005161 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/24565 | - |
dc.description.abstract | Background: Eosinophils play a key role in allergic inflammation and parasitic infections. The synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), has been previously shown to activate eosinophils and thus to enhance respiratory burst through the formyl peptide receptors. Objective: This study was undertaken to determine the intracellular signaling pathway involved in WKYMVm-stimulated superoxide production by human eosinophils. Methods: Purified eosinophils from peripheral blood were stimulated with various concentrations (10(-3) to 10 mu M) of WKYMVm and the involvement of PI3-kinase and MAP kinases in WKYMVm-triggered superoxide production was investigated using pharmacological inhibitors. Results: WKYMVm-induced superoxide production by eosinophils was strongly inhibited by pretreatment with the PI3-kinase inhibitor LY294002. In addition, pretreatment with the ERK1/2 kinase inhibitor PD98059 resulted in marked inhibition of superoxide production induced by WKYMVm. Indeed, WKYMVm strongly induced phosphorylation of ERK1/2. The ERK1/2 activation by the peptide was transient and peaked after 2 min of stimulation. Furthermore, ERK1/2 activation by WKYMVm was completely inhibited by pretreatment with the PI3-kinase inhibitor LY294002, but not by the PKC inhibitor Ro-31-8220. Conclusion: These results suggest that WKYMVm stimulates human eosinophils to induce superoxide production via a PI3-kinase-mediated ERK1/2 pathway. Copyright (C) 2005 S. Karger AG, Basel. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KARGER | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.subject | eosinophils | - |
dc.subject | WKYMVm | - |
dc.subject | superoxide | - |
dc.subject | phosphoinositide 3-kinase | - |
dc.subject | ERK1/2 | - |
dc.subject | VAL-D-MET | - |
dc.subject | HUMAN NEUTROPHILS | - |
dc.subject | HUMAN MONOCYTES | - |
dc.subject | RECEPTOR | - |
dc.subject | P47(PHOX) | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | GENERATION | - |
dc.subject | KINASE | - |
dc.subject | AKT | - |
dc.subject | HYDROLYSIS | - |
dc.title | The synthetic chemoattractant peptide WKYMVm induces superoxide production by human eosinophils via the phosphoinositide 3-kinase-mediated activation of ERK1/2 | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1159/000085428 | - |
dc.author.google | Shin, MH | - |
dc.author.google | Lee, YA | - |
dc.author.google | Bae, YS | - |
dc.author.google | Kita, H | - |
dc.author.google | Kim, K | - |
dc.author.google | Ryu, SH | - |
dc.relation.volume | 137 | - |
dc.relation.startpage | 21 | - |
dc.relation.lastpage | 26 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Conference Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, v.137, pp.21 - 26 | - |
dc.identifier.wosid | 000229643500006 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 26 | - |
dc.citation.startPage | 21 | - |
dc.citation.title | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.citation.volume | 137 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-20944433580 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.description.scptc | 8 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordPlus | VAL-D-MET | - |
dc.subject.keywordPlus | P47(PHOX) | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | 3-KINASE | - |
dc.subject.keywordAuthor | eosinophils | - |
dc.subject.keywordAuthor | WKYMVm | - |
dc.subject.keywordAuthor | superoxide | - |
dc.subject.keywordAuthor | phosphoinositide 3-kinase | - |
dc.subject.keywordAuthor | ERK1/2 | - |
dc.relation.journalWebOfScienceCategory | Allergy | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Allergy | - |
dc.relation.journalResearchArea | Immunology | - |
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