Recombinant mussel adhesive protein Mgfp-5 as a cell adhesion biomaterial
SCIE
SCOPUS
- Title
- Recombinant mussel adhesive protein Mgfp-5 as a cell adhesion biomaterial
- Authors
- Hwang, DS; Gim young soo; Kang Dong Gyun; Kim Yoen Kyu; CHA, HYUNG JOON
- Date Issued
- 2007-01-20
- Publisher
- Elsevier
- Abstract
- Mytilus galloprovincialis foot protein type-5 (Mgfp-5) is one of the mussel adhesive proteins that participate in adhesion with the substratum. We previously reported the production of recombinant Mgfp-5 in Escherichia coli and showed that the recombinant protein had superior adhesion abilities versus those of Cell-Tak, a commercially available mussel adhesive protein mixture. In the present work, we investigated the feasibility of using recombinant Mgfp-5 as a cell adhesion agent. Purified and tyrosinase-modified recombinant Mgfp-5 was used to adhere living anchorage-independent cells such as insect Drosophila S2 cells and human MOLT-4 cells onto glass slides. Our results revealed that these cell lines efficiently attached to recombinant Mgfp-5-coated glass surfaces, and that surface-immobilized S2 cells were viable and able to undergo cell division for up to 1 week. Cytochemical studies with 4',6-diamidino-2-phenylindole (DAPI) staining of nuclei and immunofluorescence for secreted foreign human erythropoietin (hEPO) from recombinant S2 cells and quantitative comparative analyses of S2 cell binding ability with Cell-Tak and poly-L-lysine, the main cell adhesion agent, were performed to demonstrate successful usage of recombinant Mgfp-5 for cell biological applications. Collectively, these results indicate that recombinant Mgfp-5 may be a useful new cell adhesion biomaterial for anchorage-independent cells. (c) 2006 Elsevier B.V. All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25694
- DOI
- 10.1016/J.JBIOTEC.2006.08.005
- ISSN
- 0168-1656
- Article Type
- Article
- Citation
- JOURNAL OF BIOTECHNOLOGY, vol. 127, no. 4, page. 727 - 735, 2007-01-20
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