DC Field | Value | Language |
---|---|---|
dc.contributor.author | Youngsoo Gim | - |
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Sunghae Lim | - |
dc.contributor.author | Younghoon Song | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2016-04-01T02:45:08Z | - |
dc.date.available | 2016-04-01T02:45:08Z | - |
dc.date.created | 2009-08-31 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.other | 2008-OAK-0000021794 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25701 | - |
dc.description.abstract | Mussel adhesive proteins (MAPs) have a potential as environmentally friendly adhesives for use under aqueous conditions. MAPs maybe of particular value in medical applications. We previously reported the functional expression of recombinant foot protein type 5 (fp-5) and foot protein type 3A (fp-3A), both of which have significant adhesion abilities, in Escherichia coli. However, these proteins were produced at low levels because of post-induction cell growth inhibition, and the proteins showed poor post-purification solubility. Here, we design and produce a new, type of recombinant MAP, fp-353, that is a fusion protein with fp-3A at each terminus of fp-5. Because fp-353 formed inclusion bodies, host cell growth inhibition did not occur. In addition, the solubility of MAP fp-353 after purification was significantly enhanced, permitting the preparation of a viscous concentrated glue solution for large-scale adhesion strength measurements. Together with large-scale cowhide adhesion measurements and cell-adhesion analyses, we successfully demonstrated that fusion mussel protein fp-353 has potential as a practical alternative bioadhesive. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.title | PRODUCTION OF FUSION MUSSEL ADHESIVE FP-353 IN ESCHERICHIA COLI | - |
dc.type | Article | - |
dc.contributor.college | 해양대학원 | - |
dc.identifier.doi | 10.1021/BP.65 | - |
dc.author.google | Gim, Y | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Lim, S | - |
dc.author.google | Song, YH | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 24 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 1272 | - |
dc.relation.lastpage | 1277 | - |
dc.contributor.id | 10167197 | - |
dc.relation.journal | BIOTECHNOLOGY PROGRESS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.24, no.6, pp.1272 - 1277 | - |
dc.identifier.wosid | 000262069100010 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1277 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1272 | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-62249159188 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 9 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CHONDROCYTE TRANSPLANTATION SURGERY | - |
dc.subject.keywordPlus | BACULOVIRAL POLYHEDRIN | - |
dc.subject.keywordPlus | POLYPHENOLIC PROTEINS | - |
dc.subject.keywordPlus | CROSS-LINKING | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PRECURSOR | - |
dc.subject.keywordAuthor | mussel adhesive protein | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.subject.keywordAuthor | fp-353 | - |
dc.subject.keywordAuthor | bioadhesive | - |
dc.subject.keywordAuthor | Mytilus galloprovincialis | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
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