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Addressable micropatterning of multiple proteins and cells by microscope projection photolithography based on a protein friendly photoresist SCIE SCOPUS

Title
Addressable micropatterning of multiple proteins and cells by microscope projection photolithography based on a protein friendly photoresist
Authors
Kim, MJong-Cheol ChoiHong-Ryul JungJoshua S.KatzMin-Gon KimDoh, J
Date Issued
2010-07-20
Publisher
ACS
Abstract
We report a new method for the micropatterning of multiple proteins and cells with micrometer-scale precision. Microscope projection photolithography based on a new protein-friendly photoresist, poly(2,2-dimethoxy nitrobenzyl methacrylate-r-methyl methacrylate-r-poly(ethylene glycol) methacrylate) (PDMP), was used for the fabrication of multicomponent protein/cell arrays. Microscope projection lithography allows precise registration between multiple patterns as well as facile fabrication of microscale features. Thin films of PDMP became soluble in near-neutral physiological buffer solutions upon UV exposure and exhibited excellent resistance to protein adsorption and cell adhesion. By harnessing advantages in microscope projection photolithography and properties of PDMP thin films, we could successfully fabricate protein arrays composed of multiple proteins. Furthermore, we could extend this method for the patterning of two different types of immune cells for the potential study of immune cell interactions. This technique will in general be useful for protein chip fabrication and high-throughput cell-cell communication study.
Keywords
T-CELLS; FLOW LITHOGRAPHY; SURFACES; ARRAYS; FEATURES
URI
https://oasis.postech.ac.kr/handle/2014.oak/25835
DOI
10.1021/LA1014253
ISSN
0743-7463
Article Type
Article
Citation
LANGMUIR, vol. 26, no. 14, page. 12112 - 12118, 2010-07-20
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도준상DOH, JUN SANG
Dept of Mechanical Enginrg
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