DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheow, LF | - |
dc.contributor.author | Ko, SH | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Kang, KH | - |
dc.contributor.author | Han, J | - |
dc.date.accessioned | 2016-04-01T02:58:29Z | - |
dc.date.available | 2016-04-01T02:58:29Z | - |
dc.date.created | 2010-04-29 | - |
dc.date.issued | 2010-04-15 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.other | 2010-OAK-0000020995 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26071 | - |
dc.description.abstract | We developed a novel method to increase the sensitivity of standard enzyme-linked immunosorbent assay (ELISA) using a multiplexed electrokinetic concentration chip. The poly(dimethylsiloxane) (PDMS) molecular concentrator(1) was used to trap and collect charged fluorescent product of target-bound enzyme turnover reaction of ELISA that occurred in a standard 96 well plate. Detection sensitivities of both prostate specific antigen (PSA) and CA 19-9 (a human pancreatic and gastrointestinal cancer marker) ELISAs in serum are enhanced similar to 100 fold with a low CV of <17%. We also integrated this method with an on-chip bead-based ELISA that lends itself toward a fully automated on-chip diagnostic device. Detection sensitivity of microfluidic bead-based CA 19-9 ELISA in serum is enhanced similar to 65 fold compared to the results without the electrokinetic accumulation step. This chip can be directly applied to enhance the readout sensitivity of a wide range of existing ELISA kits at concentrations below the current detection limit. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.subject | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject | PRECONCENTRATION | - |
dc.subject | PROTEINS | - |
dc.subject | SYSTEM | - |
dc.subject | CANCER | - |
dc.title | Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.1021/AC9024335 | - |
dc.author.google | Cheow, LF | - |
dc.author.google | Ko, SH | - |
dc.author.google | Kim, SJ | - |
dc.author.google | Kang, KH | - |
dc.author.google | Han, J | - |
dc.relation.volume | 82 | - |
dc.relation.issue | 8 | - |
dc.relation.startpage | 3383 | - |
dc.relation.lastpage | 3388 | - |
dc.contributor.id | 10107580 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.82, no.8, pp.3383 - 3388 | - |
dc.identifier.wosid | 000276557600041 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 3388 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 3383 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 82 | - |
dc.contributor.affiliatedAuthor | Kang, KH | - |
dc.identifier.scopusid | 2-s2.0-77951067248 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 49 | - |
dc.description.scptc | 44 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject.keywordPlus | PRECONCENTRATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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